Riboflavin kinase, Recombinant Human (RIFK, RP11-422N19.2, RFK)

Catalog No : USB-R2001-02
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Product name Riboflavin kinase, Recombinant Human (RIFK, RP11-422N19.2, RFK)
Catalog No USB-R2001-02
Supplier’s Catalog No R2001-02
Supplier US Biologicals
Source antigen Recombinant, E.coli
Reactivity
Cross reactivity
Applications
Molecular weight 20.5
Storage -20°C
Other names
Grade Affinity Purified
Purity ~90% by SDS-PAGE90% by SDS-PAGE Purified by immunoaffinity chromatography.
Form Supplied as liquid in 20mM Tris-HCl buffer, pH 8.0, 10% glycerol
Reactivity life 6 months
Note For reserch purpose only
Purity ~90% by SDS-PAGE90% by SDS-PAGE Purified by immunoaffinity chromatography.
Description Riboflavin kinase, also known as flavokinase, belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. It is an enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN). Recombinant riboflavin kinase , expressed in E. coli and purified by using conventional chromatography techniques. Source: Recombinant human Riboflavin kinase, 1aa-162, His-tagged, expressed in E. coli AA Sequence: MGSSHHHHHH SSGLVPRGSH MPRADCIMRH LPYFCRGQVV RGFGRGSKQL GIPTANFPEQ VVDNLPADIS TGIYYGWASV GSGDVHKMVV SIGWNPYYKN TKKSMETHIM HTFKEDFYGE ILNVAIVGYL RPEKNFDSLE SLISAIQGDI EEAKKRLELP EHLKIKEDNF FQVSKSKIMN GH Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.