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Categories: Peptides & Proteins / Proteins

Product name			Vitronectin (Vitronectin V10 Subunit, Vitronectin V65 Subunit, VN, VNT, VTN, Complement S Protein, Epibolin, S Protein, S-Protein, Serum Spreading Factor, Somatomedin B, Somatomedin-B, V75)
Catalog No			USB-V2200-05
Supplier’s Catalog No			V2200-05
Supplier			US Biologicals
Source antigen			Human plasma
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			-20°C
Other names
Grade			Affinity Purified
Purity			≥95% based on Coomassie brilliant blue stain of 10% SDS-PAGE. Human vitronectin was purified by the method of Hayman et al. using an anti-vitronectin monoclonal antibody affinity column. Purified protein sterile filtered (0.2um).
Form			Purified human vitronectin in 0.15M sodium chloride, 0.005M HEPES, pH 7.4.
Reactivity life			12 months
Note			For reserch purpose only
Purity			≥95% based on Coomassie brilliant blue stain of 10% SDS-PAGE. Human vitronectin was purified by the method of Hayman et al. using an anti-vitronectin monoclonal antibody affinity column. Purified protein sterile filtered (0.2um).
Description			Corning 24-well plates were coated with vitronectin in PBS (0.3 mL/well) at 37°C overnight. After blocking with BSA, TIG-3 cells (5 x 10e4 cells/1 mL DMEM/well) were added and incubated at 37°C for 90 minutes. Attached cells were counted electronically with a Coulter counter. Under these conditions, the use of a concentration of 3 mg vitronectin per milliliter resulted in the attachment of > 40% of the cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.