Vitronectin, Recombinant, Human
Catalog No : USB-V2200-09B
696.57€
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| Product name | Vitronectin, Recombinant, Human | ||
|---|---|---|---|
| Catalog No | USB-V2200-09B | ||
| Supplier’s Catalog No | V2200-09B | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, N-terminal, GST-tagged, full-length human Vitronectin expressed in E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 78 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~80% (SDS-PAGE and Coomassie Blue staining) | ||
| Form | Supplied as a liquid in sodium phosphate, 150mM sodium chloride, pH 7.4, 0.1mM DTT, 0.1mM PMSF, 50% glycerol. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~80% (SDS-PAGE and Coomassie Blue staining) | ||
| Description | Vitronectin is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhbits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. Extracellular matrix (ECM) proteins are produced intracellularly and are subsequently secreted into the surrounding cellular medium, actively regulating a diverse range of cell functions including cell adhesion, differentiation, proliferation, migration, invasion and survival. A primary utility of ECMs in in vitro culture is to promote cellular adhesion while maintaining cell viability and maximizing cell proliferation for downstream cell-based applications. Studies show that anchorage dependent cells growing on ECM undergo more efficient plating, have a higher proliferation rate, reach a higher density, and require lower serum and growth factor concentrations—demonstrating enhanced differentiation potential. Applications: Suitable for use in ELISA and Cell Adhesion Assay. Other applications not tested. Recommended Dilutions: Cell Adhesion Assay: Fn-/- cells (1.25x10e5 cell/cm2) were seeded on wells coated with increasing concentrations of V2200-09B. Cells were allowed to attach for 20 minutes and then washed, fixed and stained with crystal violet. Data presented as the average absorbance at 590nm of triplicate wells. Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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