Vitronectin, Recombinant, Human, Animal Free (VTN, Serum-spreading Factor, V75)
Catalog No : USB-208712
348.29€
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| Product name | Vitronectin, Recombinant, Human, Animal Free (VTN, Serum-spreading Factor, V75) | ||
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| Catalog No | USB-208712 | ||
| Supplier’s Catalog No | 208712 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, HEK293 cells | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% (SDS-PAGE gel, HPLC) | ||
| Form | Supplied as a lyophilized powder in 20mM Sodium Phosphate, pH 7.5. Reconstitute with ddH2O. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% (SDS-PAGE gel, HPLC) | ||
| Description | Vitronectin is a secreted glycoprotein which is synthesized in the liver. It circulates primarily in monomeric form, but can undergo conformational change to a structure that forms disulfide linked multimers. The multimeric Vitronectin can efficiently bind to and incorporate into the extracellular matrix. Within the matrix, Vitronectin can support cell adhesion through binding to various integrins and other proteoglycans. Additionally, recombinant vitronectin can function as a chemically defined matrix component in human embryonic stem cell renewal media. Recombinant human Vitronectin is a 459aa single chain monomeric protein, which migrates at an apparent molecular weight of 75kD by SDS-PAGE under reducing conditions. Source: Recombinant protein corresponding to human Vitronectin expressed in HEK293 cells. AA Sequence: DQESCKGRCT EGFNVDKKCQ CDELCSYYQS CCTDYTAECK PQVTRGDVFT MPEDEYTVYD DGEEKNNATV HEQVGGPSLT SDLQAQSKGN PEQTPVLKPE EEAPAPEVGA SKPEGIDSRP ETLHPGRPQP PAEEELCSGK PFDAFTDLKN GSLFAFRGQY CYELDEKAVR PGYPKLIRDV WGIEGPIDAA FTRINCQGKT YLFKGSQYWR FEDGVLDPDY PRNISDGFDG IPDNVDAALA LPAHSYSGRE RVYFFKGKQY WEYQFQHQPS QEECEGSSLS AVFEHFAMMQ RDSWEDIFEL LFWGRTSAGT RQPQFISRDW HGVPGQVDAA MAGRIYISGM APRPSLAKKQ RFRHRNRKGY RSQRGHSRGR NQNSRRPSRA TWLSLFSSEE SNLGANNYDD YRMDWLVPAT CEPIQSVFFF SGDKYYRVNL RTRRVDTVDP PYPRSIAQYW LGCPAPGHL Endotoxin: <0.1ng/ug of protein (<1EU/ug) Protein Content: Verified by UV Spectroscopy and/or SDS-PAGE gel. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with H2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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