Vitronectin, Recombinant, Human, aa1-459 (VTN, S-Protein, Serum-Spreading Factor, V75)

Catalog No : USB-298314
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Product name Vitronectin, Recombinant, Human, aa1-459 (VTN, S-Protein, Serum-Spreading Factor, V75)
Catalog No USB-298314
Supplier’s Catalog No 298314
Supplier US Biologicals
Source antigen Recombinant, Human cell line
Reactivity
Cross reactivity
Applications
Molecular weight
Storage -20°C
Other names
Grade Highly Purified
Purity ~95% (SDS-PAGE)
Form Supplied as a lyophilized powder in 20mM Tris, pH 7.5, 200mM sodium chloride. Reconstitute with sterile ddH2O.
Reactivity life 12 months
Note For reserch purpose only
Purity ~95% (SDS-PAGE)
Description Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. Source: Recombinant protein corresponding to aa1-459 from human Vitronectin, expressed serum-free in a human cell line. Molecular Weight: ~800kD (HMS), ~120kD (reduced dimer), ~65kD (monomer) Activity: Tested in cell attachment and cell spreading assays Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: with specific polyclonal antiserum Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.