SFRP3, Recombinant, Mouse (Secreted Frizzled Related Protein 3, FRZB, Fritz, Frzb1)
Catalog No : USB-S1012-88M
682.78€
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| Product name | SFRP3, Recombinant, Mouse (Secreted Frizzled Related Protein 3, FRZB, Fritz, Frzb1) | ||
|---|---|---|---|
| Catalog No | USB-S1012-88M | ||
| Supplier’s Catalog No | S1012-88M | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 34 | ||
| Storage | -70°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥ 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: ≤1.0 EU/1ug (LAL) | ||
| Form | Supplied as a lyophilized powder in PBS, pH 7.2, BSA. Reconstitute with 100ul sterile PBS. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ≥ 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: ≤1.0 EU/1ug (LAL) | ||
| Description | Secreted Frizzled Related Protein 3 (sFRP-3) was originally identified in bovine cartilage for its chondrogenic ability. Human, mouse, chick and Xenopus clones have also been isolated. sFRP-3 is often referred to as FRZB, other names also include Fritz, Frzb1, and FRP-3. At the amino acid sequence level, sFRP-3 is highly conserved. The mouse protein shares 76% identity with Xenopus, and 92% with human proteins. The gene for mouse sFRP-3 has been localized to the central region of chromosome 2. Murine sFRP-3 is expressed in the primitive streak during gastrulation, as well as in the retina, foregut diverticulum, nervous system, and posterior mesoderm during development. In adult tissues, sFRP-3 expression, as determined by Northern blot, is detected in the heart, brain, spleen, skeletal muscle, kidney and testis. The N-terminal portion of sFRP-3 protein shows 50% amino acid identity to the corresponding region of the Drosophila frizzled gene product, a receptor for Wg/Wnt signals. The similarity of sFRP-3 with frizzled proteins is restricted to the N-terminal cysteine-rich domain (CRD) that contains at least ten cysteine residues with highly conserved spacing between them. sFRP-3 was subsequently shown to be a soluble antagonist of Wnt signals. It lacks all transmembrane domains of frizzled proteins but retains the ability to bind Wnts. Ectopic expression of sFRP-3 mRNA has been shown to interfere with the induction of secondary axes in Xenopus embryos injected with Xwnt-8 mRNA (Hoang et al., 1996, J. Biol. Chem. 271:26131; Leyns et al., 1997, Cell 88:747; Wang et al., 1997, Cell 88:757; Mayr et al., 1997, Mech. Dev. 63:109; Rattner et al., 1997, PNAS 94:2859). A DNA sequence encoding the mature mouse sFRP-3 protein sequence Ala33-Ser323 was fused to a 6X histidine tag at the carboxy-terminus. The fusion protein was expressed in a mouse myeloma cell line, NS0. Swiss/UniProt Accession: P97401 Molecular Mass: Based on N-terminal sequencing, the mature mouse sFRP-3 protein starts at Ala33 and has a calculated molecular mass of 34kD. As a result of glycosylation, the recombinant protein migrates as an ~37kD protein in SDS-PAGE under reducing conditions. Activity: Measured by its ability to inhibit proliferation in MC3T3-E1 mouse preosteoblast cells. ED50 1.5-6.0ug/ml. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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