WISP1 (Wnt 1 induced Secreted Protein 1, Wnt-1-induced Secreted Protein 1, Wnt1-induced Secreted Protein 1, WISP 1, WISP-1, CCN4, WISP1c, WISP1i, WISP1tc, Wnt-1-induced Secreted Protein, Wnt1-induced Secreted Protein, Wnt-1-inducible Signaling Pathway Protein 1, Wnt1-inducible Signaling Pathway Protein 1, Wnt 1 Signaling Pathway Protein 1, Wnt1 Signaling Pathway Protein 1, Wnt-1 Signaling Pathway Protein 1) (Control Peptide)
Catalog No : USB-W1450-50A-P
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| Product name | WISP1 (Wnt 1 induced Secreted Protein 1, Wnt-1-induced Secreted Protein 1, Wnt1-induced Secreted Protein 1, WISP 1, WISP-1, CCN4, WISP1c, WISP1i, WISP1tc, Wnt-1-induced Secreted Protein, Wnt1-induced Secreted Protein, Wnt-1-inducible Signaling Pathway Protein 1, Wnt1-inducible Signaling Pathway Protein 1, Wnt 1 Signaling Pathway Protein 1, Wnt1 Signaling Pathway Protein 1, Wnt-1 Signaling Pathway Protein 1) (Control Peptide) | ||
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| Catalog No | USB-W1450-50A-P | ||
| Supplier’s Catalog No | W1450-50A-P | ||
| Supplier | US Biologicals | ||
| Source antigen | Synthetic | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | Highly Purified | ||
| Form | Supplied as a lyophilized powder in PBS. Reconstitution: Reconstitute with 200ul sterile ddH2O. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Highly Purified | ||
| Description | C-ESYPDFSEIAN, from the C-Terminus of human WISP1. Control peptide corresponding to polyclonal antibody, Catalog#W1450-50A, goat x human. This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Alternative splicing of this gene generates 2 transcript variants. Applications: Suitable for use in ELISA. Other Applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking (see corresponding antibody W1450-50A): Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel. | ||
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