Wnt4 (Wnt4, Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member 4, Wnt-4, Wnt-4 Protein Precursor) (Control Peptide)
Catalog No : USB-W3021-01-P
421.85€
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| Product name | Wnt4 (Wnt4, Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member 4, Wnt-4, Wnt-4 Protein Precursor) (Control Peptide) | ||
|---|---|---|---|
| Catalog No | USB-W3021-01-P | ||
| Supplier’s Catalog No | W3021-01-P | ||
| Supplier | US Biologicals | ||
| Source antigen | Synthetic | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | Highly Purified | ||
| Form | Supplied as a lyophilized powder in PBS. Reconstitution: Reconstitute with 200ul sterile ddH2O. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | Highly Purified | ||
| Description | C-SNWLYLAKLSSVGS, internal region Control peptide corresponding to polyclonal antibody, Catalog#W3021-01, goat x human. Application: Suitable for use in ELISA and Antibody Blocking. Other applications not tested. Recommended Diluiton: Antibody Blocking: 1:1 1ug antibody/1ug peptide Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking (see corresponding antibody W3021-01): Antibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel. | ||
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