Frizzled 8, Fc Chimera, Recombinant, Human (FZD8, Frizzled-8, Fz-8, hFz8)
Catalog No : USB-F6401-39A
682.78€
0.00€
Shipping cost plus VAT not included , delivery in 7-14 business days
| Product name | Frizzled 8, Fc Chimera, Recombinant, Human (FZD8, Frizzled-8, Fz-8, hFz8) | ||
|---|---|---|---|
| Catalog No | USB-F6401-39A | ||
| Supplier’s Catalog No | F6401-39A | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, Human Frizzled 8 (Ala25-Pro172) at the N-terminus, IEGRMD and human IgG1 (Pro100-Lys330) at the C-terminus derived in Chinese Hamster Ovary cell line. | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 43 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~95% as determined by SDS-PAGE and visualized by silver stain. | ||
| Form | Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile PBS to 0.5mg/ml. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~95% as determined by SDS-PAGE and visualized by silver stain. | ||
| Description | Frizzled-8 is one of at least ten seven- transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors. Frizzled proteins are thought to be G-protein-coupled. Wnt engagement, with low density lipoprotein receptor- related proteins LRP-5 or LRP-6 acting as co-receptors, stabilizes b-catenin and promotes gene transcription that is important in development and tissue maintenance. Human Frizzled-8 cDNA encodes 694aa including a 27aa signal peptide, a 247aa extracellular domain (ECD), the 7TM region (aa276-605), and a C-terminal cytoplasmic domain with a PDZ binding motif (aa606-694). The ECD includes a cysteine-rich region (CRD, aa30-151) that binds Wnts and is highly conserved among Frizzleds, and a linker region. Within aa28-172, human Frizzled-8 shares 99%, 99%, 90% and 85% aa identity with mouse, rat, Xenopus and zebrafish Frizzled-8, respectively. It also shares 82% aa identity with human Frizzled-5. Frizzleds can form homodimers or selective hetero-oligomers with other family members, which can involve the TM regions and possibly the CRD. During mouse development, Frizzled-8 is expressed in tissues that are important for organizing the anterior-posterior axis. Interactions of Frizzled-8 with several non-Wnt ligands have been identified. Interaction with Frizzled-8 and LRP-6 allows R-spondins to activate b-catenin signaling pathways, while interaction with IGFBP-4 (insulin-like growth factor binding protein or CTGF (connective tissue growth factor) inhibits Wnt signaling. These ligands bind the extracellular CRD of Frizzled-8, blocking Wnt binding. The recombinant Frizzled-8 CRD has also been used to block Wnt signaling and inhibit growth of teratocarcinomas. SDS-PAGE: 55-65kD, reducing conditions Activity: Measured by its binding ability in a functional ELISA. In a 100ul reaction mixture containing biotinylated recombinant mouse Wnt-3a or Wnt-5a at 50-100ng/ml and recombinant human Frizzled 8/Fc Chimera dilutions at 0.1-8000ng/ml, the concentration of recombinant human Frizzled 8/Fc Chimera that produces 50% of the optimal binding response is found to be ~7-28ng/ml.l. Endotoxin Level: ≤1EU/1ug (LAL) Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
Product Details
© 2020 Imugex All Rights Reserved