Wnt10b, Recombinant, Mouse (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member 10B, Wnt 10b, Wnt-10b, Wnt12)
Catalog No : USB-W3038-10H
740.25€
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| Product name | Wnt10b, Recombinant, Mouse (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member 10B, Wnt 10b, Wnt-10b, Wnt12) | ||
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| Catalog No | USB-W3038-10H | ||
| Supplier’s Catalog No | W3038-10H | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, CHO | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 40.1 | ||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Purified | ||
| Purity | ~85% (SDS-PAGE) | ||
| Form | Supplied as a lyophilized powder in PBS, EDTA, CHAPS. Reconstitute with PBS to 100ug/ml. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Purity | ~85% (SDS-PAGE) | ||
| Description | Wnt-10b (also known as Wnt-12) is a 42-44kD member of the Wnt family of secreted, highly conserved, cysteine-rich glycoproteins that play important roles in vertebrate pattern formation, cell fate decision, axon guidance, and tumor formation (1-3). Mouse Wnt-10b cDNA encodes a 361aa precursor that contains a 28 aa signal sequence plus a 361 aa mature protein that contains two glycosylation sites, three potential phosphorylation sites, and a potential palmitoylation site (3, 4). A short isoform is reported that lacks aa 143 - 238 of the precursor (1, 4). Mouse Wnt-10b shares 97-98% aa identity with human, rat, equine, porcine and canine Wnt-10b. Wnt-10b plays a critical role in maintaining mesenchymal stem cells and determining whether they differentiate to adipocytes or osteoblasts (5-7). Mouse Wnt-10b deletion produces age-dependent loss of bone mass due to defective production of osteoblasts, while transgenic over-expression increases postnatal osteoblast differentiation and inhibits adipocyte differentiation (5-7). Ectopic expression of Wnt-10b in an obesity and diabetes-prone background, such as the ob/ob mouse, inhibits obesity (8). In mouse skeletal muscle, Wnt-10b is expressed inversely with SREBP1c and increases insulin sensitivity (9). In humans, a mis-sense polymorphism is responsible for a malformation of hands and feet, while a C256Y inactivating mutation is associated with severe early-onset obesity (10, 11). Wnt-10 is mainly produced by stem cells and pre-osteoblasts, but also by adult bone marrow CD8+ T lymphocytes stimulated with parathyroid hormone (12). In some hepatocellular carcinomas, Wnt-10b can inhibit cancer cell growth, but in others, it can act synergistically with FGFs to stimulate cell growth (13). Several Wnts, including Wnt-10b, are expressed in both normal and/or malignant colon tissues (14). As a key regulator, Wnt signaling plays a major role in the process of colon carcinogenesis (15). Source: Recombinant corresponding to aa1-389 of mouse Wnt-10b, expressed in CHO cells. Molecular Weight: ~40.1kD Endotoxin Level: ≤1EU/1ug (LAL) Biological Activity: Measured in a cell proliferation/survival assay using IEC-18 rat small intestinal epithelial cell line (ATCC: CRL-1589). The ED50 for this effect is typically 0.9-3.6ug/ml. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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