WNT-1, Recombinant, Human (WNT-1 Proto-Oncogene Protein, INT1, WNT1)
Catalog No : USB-215213
621.86€
0.00€
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| Product name | WNT-1, Recombinant, Human (WNT-1 Proto-Oncogene Protein, INT1, WNT1) | ||
|---|---|---|---|
| Catalog No | USB-215213 | ||
| Supplier’s Catalog No | 215213 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 38.4 | ||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ~98% (SDS-PAGE, HPLC) | ||
| Form | Supplied as a lyophilized powder in PBS. No preservative added. Reconstitute with 100ul sterile dH2O. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Purity | ~98% (SDS-PAGE, HPLC) | ||
| Description | Wnt-1, formerly known as int-1, is a non-glycosylated secreted protein associated with the extracellular matrix, involved in normal embryonic development and particularly linked with breast cancer. Wnt-1, which signals through binding to the Frizzled family of transmembrane receptors, was originally identified as a proto-oncogene implicated in carcinomas induced by the mouse mammary tumor virus (MMTV). Wnt-1 belongs to a family of Wnt genes involved in the dorso-ventricular development of the mesoderm during embryogenesis, with a restricted temporal and spatial pattern of expression. Source: Recombinant protein corresponding to human WNT-1, expressed in E. coli. Molecular Weight: ~38.4kD (343aa residues). Biological Activity: The ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5-2.5ng/ml in the presence of 200ng/ml of human BMP-2. Applications: Suitable for use in Functional Assays. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Endotoxin: <1EU/ug Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with 100ul sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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