Wnt3a, Recombinant, Human (Protein Wnt-3a, Wingless-Type MMTV Integration Site Family, Member 3A, WNT3A)

Catalog No : USB-301573
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Product name Wnt3a, Recombinant, Human (Protein Wnt-3a, Wingless-Type MMTV Integration Site Family, Member 3A, WNT3A)
Catalog No USB-301573
Supplier’s Catalog No 301573
Supplier US Biologicals
Source antigen Recombinant, CHO
Reactivity
Cross reactivity
Applications
Molecular weight 41
Storage -20°C
Other names
Grade Purified
Purity ~75% (SDS-PAGE)
Form Supplied as a liquid in Tris-HCl, pH 7.2-7.4, 0.5% CHAPS, 0.1% BSA.
Reactivity life 12 months
Note For reserch purpose only
Purity ~75% (SDS-PAGE)
Description Protein Wnt-3a is a protein that is encoded by the WNT3A gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis, adipogenesis, etc. and in several other developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Human Wnt3a shows 96% amino acid identity to mouse Wnt3a protein. Source: Recombinant protein corresponding to aa19-352 of human Wnt3a, CHO cell line-derived. Molecular Weight: ~41kD Biological Activity: Wnt3a activity has been measured using TCF-based Wnt reporter stable cell lines (HEK293 and NIH3T3). 4ng/ml of Wnt3a generate fourteen- to twenty-fold increase of luciferase activity compared to control (buffer without Wnt3a). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.