Wnt inhibitory Factor 1, Recombinant, Mouse, aa29-379, His-Tag (Wif-1, WIF-1)

Catalog No : USB-351578
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Product name Wnt inhibitory Factor 1, Recombinant, Mouse, aa29-379, His-Tag (Wif-1, WIF-1)
Catalog No USB-351578
Supplier’s Catalog No 351578
Supplier US Biologicals
Source antigen Recombinant, mouse from insect cell line
Reactivity
Cross reactivity
Applications
Molecular weight 39.4
Storage -20°C/-70°C
Other names
Grade Purified
Purity ~90% (SDS-PAGE)
Form Supplied as a liquid in 20mM MES, pH 5.5, 1mM DTT, 1mM PMSF, 30% glycerol.
Reactivity life 12 months
Note For reserch purpose only
Purity ~90% (SDS-PAGE)
Description WIF1, also known as wnt inhibitory factor 1, is a secreted protein that binds to Wnt proteins and inhibits their activities. This protein signaling plays a pivotal role in skeletal development and in the control of cartilage and bone turnover. Also, WIF1 is present in fish, amphibia and mammals, and is expressed during Xenopus and zebrafish development in a complex pattern that includes paraxial presomitic mesoderm, notochord, branchial arches and neural crest derivatives. Source: Recombinant protein corresponding to aa29-379 from mouse WIF1, fused to His-tag at C-terminal expressed in insect cell. Molecular Weight: ~39.4kD (359aa) AA Sequence: GQPPEESLYL WIDAHQARVL IGFEEDILIV SEGKMAPFTH DFRKAQQRMP AIPVNIHSMN FTWQAAGQAE YFYEFLSLRS LDKGIMADPT VNVPLLGTVP HKASVVQVGF PCLGKQDGVA AFEVNVIVMN SEGNTILRTP QNAIFFKTCQ QAECPGGCRN GGFCNERRVC ECPDGFYGPH CEKALCIPRC MNGGLCVTPG FCICPPGFYG VNCDKANCST TCFNGGTCFY PGKCICPPGL EGEQCELSKC PQPCRNGGKC IGKSKCKCPK GYQGDLCSKP VCEPGCGAHG TCHEPNKCQC REGWHGRHCN KRYGASLMHA PRPAGAGLER HTPSLKKAED RRDPPESNYI WVEHHHHHH Endotoxin: ~1EU/1ug (LAL) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.