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Categories: Peptides & Proteins / Proteins

Product name			WNT1, Recombinant, Human (Proto-Oncogene Int-1 Homolog, Wnt-1, Proto-Oncogene Wnt-1)
Catalog No			USB-500550
Supplier’s Catalog No			500550
Supplier			US Biologicals
Source antigen			Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			38.5

Storage			-20°C
Other names
Grade			Highly Purified
Purity			~95% (SDS-PAGE)
Form			Supplied as a lyophilized powder in Tris, DTT. Reconstitute with sterile ddH2O to 0.1mg/ml.
Reactivity life			6 months
Note			For reserch purpose only
Purity			~95% (SDS-PAGE)
Description			Wnt-1 is a secreted protein that signals through the frizzled family of seven transmembrane receptors. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the up-regulation of over fifty genes. Stabilization and resulting accumulation of the intracellular signaling protein beta-catenin is an important aspect of Wnt-1 signaling. While known to play in role as a developmental protein, elevated levels of Wnt proteins have also been associated with tumorigenesis. Source: Recombinant protein corresponding to human WNT1 expressed in E. coli. Molecular Weight: ~38.5kD Biological Activity: Determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5-2.5ng/ml in the presence of 200ng/ml of human BMP-2. Endotoxin: <1EU/ug (LAL) Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.