Streptavidin (Beta Galactosidase)
Catalog No : USB-S7973-79
568.98€
0.00€
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| Product name | Streptavidin (Beta Galactosidase) | ||
|---|---|---|---|
| Catalog No | USB-S7973-79 | ||
| Supplier’s Catalog No | S7973-79 | ||
| Supplier | US Biologicals | ||
| Source antigen | |||
| Reactivity | |||
| Cross reactivity | |||
| Applications | FLISA, IP, WB | ||
| Molecular weight | |||
| Storage | 4°C Do Not Freeze | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | Purified by ion exchange chromatography. Assay by IEP demonstrated a single precipitin arc against anti-beta galactosidase (E. coli) and anti-streptavidin. No crossreactivity was observed against anti-avidin. | ||
| Form | Supplied as a liquid in PBS, 0.01M magnesium chloride, pH7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Beta Galactosidase (E. coli) (MW: 464kD). | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Description | Streptavidin is a biotin-binding protein found in the culture broth of the bacterium Streptomyces avidinii. Like its namesake avidin, streptavidin binds 4 moles of biotin per mole of protein with a high affinity virtually unmatched in nature (Kd~10-15). Streptavidin lacks carbohydrate side chains present on avidin and has an isoelectric point nearer to neutrality where most useful biological interactions occur (pI of 5-6 vs 10 for avidin). As a result, streptavidin frequently exhibits lower levels of non-specific binding than does avidin when the proteins are used in applications relying upon the formation of avidin/biotin complexes (Chaiet and Wolf, 1964). Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:100-1:3000 Assayed against 1ug of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at RT. Optimal dilutions to be determined by researcher. Storage and Stability: Store at 4° C before opening. DO NOT FREEZE. This product is stable at 4°C as an undiluted liquid. Stable for 6 months. Dilute only prior to immediate use. Freezing Beta Galactosidase conjugates will result in a substantial loss of enzymatic activity. | ||
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