Streptavidin (HRP)
Catalog No : USB-S7973-97A
389.67€
0.00€
Shipping cost plus VAT not included , delivery in 7-14 business days
| Product name | Streptavidin (HRP) | ||
|---|---|---|---|
| Catalog No | USB-S7973-97A | ||
| Supplier’s Catalog No | S7973-97A | ||
| Supplier | US Biologicals | ||
| Source antigen | Streptomyces avidinii | ||
| Reactivity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-streptavidin. No reaction was observed against anti-avidin. | ||
| Cross reactivity | |||
| Applications | FLISA, IP, WB | ||
| Molecular weight | |||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Purified | ||
| Purity | Purified by ion exchange chromatography. | ||
| Form | Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease Free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP). | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Description | Streptavidin is a non-glycosylated polypeptide chain having a molecular mass of 240kD and containg 4 subunits each with a binding site for biotin. The neutral isoelectric point of Streptavidin and the high affinity of the biotin-streptavidin interaction result in a very quick reaction with a high signal to noise ratio. Streptavidin does not react non-specifically with endogenous lectins when used in immunological assays on cells and tissues. Streptavidin is non- or very low charged around pH 7, reducing NSB to charged molecules to a very limited level. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:20,000-1:200,000 Western Blot: 1:10,000-1:40,000 Immunohistochemistry: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light. | ||
Product Details
© 2020 Imugex All Rights Reserved