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Categories: Biochemicals / Biochemicals

Product name			Streptavidin (PE)
Catalog No			USB-S7973-92F2
Supplier’s Catalog No			S7973-92F2
Supplier			US Biologicals
Source antigen			S. avidinii
Reactivity			Demonstrates negligible non-specific binding to non-biotinylated macromolecules. Gives very low backgrounds.
Cross reactivity
Applications			FLISA, IP, WB
Molecular weight

Storage			4°C Do Not Freeze
Other names
Grade			Purified
Purity			Purified by medium pressure liquid chromatography on gel filtration columns of AcA34 ultrogel.
Form			Supplied as a lyophilized powder in PBS, 0.09% sodium azide, 1% BSA. Labeled with Phycoerythrin (PE). Reconstitute with 1ml sterile dH2O.
Reactivity life			12 months
Note			For reserch purpose only
Description			Streptavidin labeled with PE. Preparation: Pure Streptavidin is reacted with Succinimidyl 4- (N-maleimido-methyl) cyclohexane-1- carboxylate (SMCC). R. Phycoerythrin is reacted with N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP) and activated by reduction with dithiothreitol. The two substitiuted proteins are reacted together to give covalent conjugates, which are selected by medium pressure liquid chromatography on gel filtration columns of AcA34 ultrogel. Conjugates show negligible non-specific binding to non-biotinylated macromolecules, and therefore gives very low backgrounds. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Neat. Use 10ul to label 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher. Storage and Stability: Stored at 4°C. Do not freeze. Protect from light. Aliquots are stable for at least 12-12 months at 4°C