Streptavidin (HRP)
Catalog No : USB-S7973-97B
498.86€
0.00€
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Product name | Streptavidin (HRP) | ||
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Catalog No | USB-S7973-97B | ||
Supplier’s Catalog No | S7973-97B | ||
Supplier | US Biologicals | ||
Source antigen | Horseradish peroxidase | ||
Reactivity | Negligible non-specific binding to non-biotinylated macromolecules. Giving very low backgrounds. | ||
Cross reactivity | |||
Applications | FLISA, IP, WB | ||
Molecular weight |
Storage | -20°C | ||
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Other names | |||
Grade | Purified | ||
Purity | Purified | ||
Form | Supplied as a liquid with 0.01% Thimerosal. Labeled with horseradish peroxidase (HRP). | ||
Reactivity life | 12 months | ||
Note | For reserch purpose only | ||
Description | Streptavidin is a non-glycosylated polypeptide chain having a molecular mass of 240kD and containg 4 subunits each with a binding site for biotin. The neutral isoelectric point of Streptavidin and the high affinity of the biotin-streptavidin interaction result in a very quick reaction with a high signal to noise ratio. Streptavidin does not react non-specifically with endogenous lectins when used in immunological assays on cells and tissues. Streptavidin is non- or very low charged around pH 7, reducing NSB to charged molecules to a very limited level. Applications: Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Frozen/paraffin): 1:500 ELISA: 1:1000-1:10,000 Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light. |
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