Streptavidin (Sepharose 4B)
Catalog No : USB-S7973-89E
327.60€
0.00€
Shipping cost plus VAT not included , delivery in 7-14 business days
| Product name | Streptavidin (Sepharose 4B) | ||
|---|---|---|---|
| Catalog No | USB-S7973-89E | ||
| Supplier’s Catalog No | S7973-89E | ||
| Supplier | US Biologicals | ||
| Source antigen | Streptocyces avidinii | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | FLISA, IP, WB | ||
| Molecular weight | |||
| Storage | 4°C Do Not Freeze | ||
|---|---|---|---|
| Other names | |||
| Grade | Purified | ||
| Purity | |||
| Form | Supplied as suspension in buffered saline solution. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Description | Streptavidin (MW ~66kD) binds specifically with biotin (244D). It is derived from the bacterium Streptomyces avidinii and bears a remarkable similarity to chicken egg-white avidin both in three-dimensional structure and its ability to bind biotin with extremely high affinity (Kd=10e-15M). It is a tetrameric protein capable of binding up to 4 biotin molecules. Unlike avidin, Streptavidin is non-glycosylated and is essentially neutral in charge, whereas avidin (pH 10.5) is basic at neutral pH. Because of this, streptavidin has considerably less non-specific binding resulting in less background. It has replaced avidin as the reagent of choice for most applications where protein interactions may cause background. Streptavidin Sepharose: The same high performance streptavidin media used in Streptavidin columns for fast, reliable purification of biotinylated compounds. Ideal for packing into columns when larger scale purifications are required. Extremely useful for exploiting either the strong interactions of biotin and streptavidin or the somewhat weaker interactions of 2-iminobiotin and streptavidin. The base matrix is a rigid, highly cross-linked beaded agarose with high chemical stability. The immobilized streptavidin binds biotin and biotinylated substances and can be used for affinity chromatography applications. The interaction between streptavidin and biotin is very strong and requires denaturating conditions for elution, which may destroy both the ligand and the sample. Alternatively, it can be used in the purification of antigens, where biotinylated antibodies are incubated with antigen. The biotinylated antibody-antigen complex binds to Streptavidin Sepharose from which the antigen can be eluted. Another example is to utilise the interaction between 2-iminobiotin and streptavidin, eluting the bound substances at pH 4. Matrix: Highly cross-linked agarose 4% Bead Size: 52-165um Binding Capacity: >20ug biotin/ml drained gel Exclusion Limit: ~2x10e7 pH Stability: 3-10 Matrix Activation: Cyanogen bromide Binding Buffer: 50mM ammonium carbonate, 0.5M NaCl, pH10 Elution Buffer: 50mM ammonium acetate, 0.5M NaCl, pH4 Applications: Suitable for use in Affinity Chromatography. Storage and Stability: Store in 20% ethanol. May be stored at 4°C. Do not freeze. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. | ||
Product Details
© 2020 Imugex All Rights Reserved