Avidin (Egg White, 68kD Glycoprotein)

Catalog No : USB-A4500-02
377.02€
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Product name Avidin (Egg White, 68kD Glycoprotein)
Catalog No USB-A4500-02
Supplier’s Catalog No A4500-02
Supplier US Biologicals
Source antigen Hen egg white
Reactivity
Cross reactivity
Applications
Molecular weight 68
Storage -20°C
Other names
Grade Affinity Purified
Purity Purified by affinity chromatographic techniques.
Form Supplied as a lyophilized powder.
Reactivity life 12 months
Note For reserch purpose only
Description Avidin is a glycosylated polypeptide chain having a molecular mass of 68kD and containg 4 subunits each with a binding site for biotin. Avidin is purified by affinity chromatographic techniques. The purification procedure ensures minimal contamination by other proteins or DNA. The resulting high activity and purity of the product gives very low non-specific binding (NSB). Activity: Greater than 11 U/mg, 1 unit binds 1µg biotin. Endotoxin: ≤ 0.1ng/ug (IEU/ug) of Avidin. Application: Avidin may be used to visualize biotin conjugated molecules in ELISA, blotting and histological techniques. Solubility: It is recommended to reconstitute the lyophilized Avidin in sterile 18MΩ-cm H2O not less than 100µg/ml or more than 10mg/ml. solutions Stability: Avidin although stable at 40C for 3 weeks, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.