Histone Deacetylase, Assay Buffer (HDAC)
Catalog No : USB-H5109-66A
370.13€
0.00€
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| Product name | Histone Deacetylase, Assay Buffer (HDAC) | ||
|---|---|---|---|
| Catalog No | USB-H5109-66A | ||
| Supplier’s Catalog No | H5109-66A | ||
| Supplier | US Biologicals | ||
| Source antigen | |||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -70°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥ 95% by HPLC | ||
| Form | Supplied as a liquid in TBS. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Description | Buffer essential for use with the HDAC assay of purified HDACs. Usage: In a 50ul reaction, assay HDAC activity using HDAC (enzyme, lysate, etc.), substrate, HDAC assay buffer, and test inhibitor. Incubate microtiter plate at 37°C for 30 minutes. Add an equal volume (50ul) of HDAC Assay Developer to each well. Incubate the plate at room temperature for 15 minutes. Read sample in a microtiter-plate reading fluorimeter capable of excitation at a wavelength in the range of 350-380nm and detection of emitted light in the range of 440-460nm. “Blank” value (no enzyme negative control) is subtracted from all other values. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months. | ||
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