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Categories: Biochemicals / Biochemicals

Product name			Streptavidin, His-Tag, 25-183aa, Recombinant, Human
Catalog No			USB-S7973-73B
Supplier’s Catalog No			S7973-73B
Supplier			US Biologicals
Source antigen			Streptomyces avidinii, His tagged, Recombinant, E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			17

Storage			-20°C
Other names
Grade			Highly Purified
Purity			~90% by SDS-PAGE
Form			Supplied as a liquid in 20mM Tris pH 7.5.
Reactivity life			12 months
Note			For reserch purpose only
Description			Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant His tagged streptavidin was expressed in E.coli and purified by using conventional chromatography techniques. Sequence: MVHHHHHHDP SKDSKAQVSA AEAGITGTWY NQLGSTFIVT AGADGALTGT YESAVGNAES RYVLTGRYDS APATDGSGTA LGWTVAWKNN YRNAHSATTW SGQYVGGAEA RINTQWLLTS GTTEANAWKS TLVGHDTFTK VKPSAASIDA AKKAGVNNGN PLDAVQQ Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.