Protein L, Recombinant, Peptostreptococcus Magnus
Catalog No : USB-P9106D
402.31€
0.00€
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| Product name | Protein L, Recombinant, Peptostreptococcus Magnus | ||
|---|---|---|---|
| Catalog No | USB-P9106D | ||
| Supplier’s Catalog No | P9106D | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E. coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 43.2 | ||
| Storage | -20°C | ||
|---|---|---|---|
| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥98% (SDS-PAGE and HPLC) | ||
| Form | Supplied as a lyophilized powder. Reconstitute with ddH2O to a concentration of 5mg/ml. | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Description | Protein L has the unique ability to bind through kappa light chain interactions without interfering with the antibody's antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. Protein L can be used to detect, quantify and purify antibodies and antibody/antigen complexes. United States Biological's Recombinant Protein L contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity. The 6-His-tag on N-terminus can be used for affinity purification or protein L detection using anti-His-tag antibodies. Protein L binds to all classes of Ig (IgG, IgM, IgA, IgE, and IgD). Protein L will also bind Single Chain Variable Fragments (ScFv) and Fab Fragments. Protein L binds kappa I, III, and IV in human and kappa I on mouse. SOURCE: E. coli SEQUENCE: Amino acid 98-462 of the Peptostreptococcus Magnus. Protein L Contains 5 kappa-binding domain with 6x His-tag on N-terminus. Gene Bank Accession Number CAA25612. Endotoxin level: ≤0.1EU/mg Specificity: Under optimal conditions, 1mg protein L will bind approximately 5mg human IgG. Optimal binding of Protein L to antibodies occurs at pH 7.5 over a pH range of 2.5 to 3.0. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Stable for at least 6 months at -20°C. Reconstitute with sterile ddH2O, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer | ||
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