Streptavidin (HRP)

Catalog No : USB-S7973-75D
787.38€
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Product name Streptavidin (HRP)
Catalog No USB-S7973-75D
Supplier’s Catalog No S7973-75D
Supplier US Biologicals
Source antigen Horseradish peroxidase
Reactivity
Cross reactivity
Applications
Molecular weight
Storage -20°C
Other names
Grade Purified
Purity Purified
Form Supplied as a liquid in mercury free stabilized solution, 10ppm ProclinTM 300, 0.01% methylisothiazolone, 0.01% bromonitrodioxane. Labeled with horseradish peroxidase (HRP).
Reactivity life 12 months
Note For reserch purpose only
Description Streptavidin is a non-glycosylated polypeptide chain having a molecular mass of 240kD and containg 4 subunits each with a binding site for biotin. The Streptavidin is isolated by purification from fermentation broth. The neutral isoelectric point of Streptavidin and the high affinity of the biotin-streptavidin interaction result in a very quick reaction with a high signal to noise ratio. Streptavidin does not react unspecifically with endogenous lectins when used in immunological assays on cells and tissues. Streptavidin is non- or very low charged around pH 7, reducing NSB to charged molecules to a very limited level. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.