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Categories: Biochemicals / Biochemicals

Product name			Streptavidin (AP) (Streptomyces avidinii)
Catalog No			USB-S7973-77A
Supplier’s Catalog No			S7973-77A
Supplier			US Biologicals
Source antigen			Streptomyces avidinii
Reactivity
Cross reactivity
Applications
Molecular weight			240

Storage			4°C Do Not Freeze
Other names
Grade			Purified
Purity			Purified. Calf intestinal alkaline phosphatase conjugated to Streptavidin (Streptomyces avidinii).
Form			Supplied as a liquid in conjugate stabilizing buffer, 0.01% methylisothiazolone, 0.01% bromonitrodioxane. Labeled with AP.
Reactivity life			12 months
Note			For reserch purpose only
Description			Streptavidin is a non-glycosylated polypeptide chain having a molecular mass of 240kD and containg 4 subunits each with a binding site for biotin. The Streptavidin is isolated by purification from fermentation broth. The neutral isoelectric point of Streptavidin and the high affinity of the biotin-streptavidin interaction result in a very quick reaction with a high signal to noise ratio. Streptavidin does not react unspecifically with endogenous lectins when used in immunological assays on cells and tissues. Streptavidin is non- or very low charged around pH 7, reducing NSB to charged molecules to a very limited level. Source: Streptomyces avidinii Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.