B-Galactosidase (Biotin)
Catalog No : USB-168054
501.16€
0.00€
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| Product name | B-Galactosidase (Biotin) | ||
|---|---|---|---|
| Catalog No | USB-168054 | ||
| Supplier’s Catalog No | 168054 | ||
| Supplier | US Biologicals | ||
| Source antigen | |||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | 4°C Do Not Freeze | ||
|---|---|---|---|
| Other names | |||
| Grade | Purified | ||
| Purity | Purified | ||
| Form | Supplied as a liquid in 0.05M potassium phosphate, 0.001M magnesium chloride, 0.1M beta-mercaptoethanol, pH 7.8. Labeled with Biotin. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Description | Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin; an attraction that is the strongest and most stable non-covalent interaction known. B-galactosidase (b-gal; b-gal) is an enzyme that hydrolyzes b-galactosides (such as lactose and various glycoproteins) into simple sugars. B-galactosidase is routinely used as a reporter in biochemical experiments as well as a marker for gene expression experiments in E. coli. B-galactosidase can be used to cleave the chemical X-gal that forms an intense blue product that can easily be quantified. Source: B-Galactosidase, expressed in E.coli. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:8000-1:32,000 Western Blot: 1:500-1:2500 Immunohistochemistry: 1:200-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at 4°C. Do Not Freeze Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | ||
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