Imugex

Shipping cost plus VAT not included , delivery in 7-14 business days

Categories: Biochemicals / Biochemicals

Product name			Green Fluorescent Protein, Recombinant (GFP)
Catalog No			USB-G8965-10E
Supplier’s Catalog No			G8965-10E
Supplier			US Biologicals
Source antigen			E. coli
Reactivity
Cross reactivity
Applications
Molecular weight			28

Storage			-20°C
Other names
Grade			Purified
Purity			Purified using Ni-NTA agarose (~70%).
Form			Supplied as a liquid in PBS, 20% glycerol.
Reactivity life			12 months
Note			For reserch purpose only
Description			GFP is a 27kD monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (395nm) and emits green light (508nm) in the absence of additional proteins, substrates, or co-factors. Intact, native GFP protein is required for fluorescence. The GFP chromophore results from the cyclization and oxidation of the sequence Ser65-Tyr66-Gly67. GFP fluorescence is stable, species independent, and suitable for a variety of applications. Recombinant protein corresponding to full-length Green Fluorescent Protein with N-terminal His-tag, expressed in E. coli. Accession Number: AAA27722 Applications: Suitable for use as a standard for protein gels and Western Blots (~28kD). Also suitable for calibration of ﬂuorometers and microinjection into cells and tissues. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.