Noelin-1, Recombinant, Mouse (Neuronal Olfactomedin-related Endoplasmic Reticulum-localized-1, Olfactomedin-1, Pancortin)
Catalog No : USB-N3040
682.78€
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| Product name | Noelin-1, Recombinant, Mouse (Neuronal Olfactomedin-related Endoplasmic Reticulum-localized-1, Olfactomedin-1, Pancortin) | ||
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| Catalog No | USB-N3040 | ||
| Supplier’s Catalog No | N3040 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, NSO cells | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥ 90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: ≤ 1EU/1ug. | ||
| Form | Supplied as a lyophilized powder from PBS, pH 6.5. Reconstitute with sterile PBS to a concentration of ≥100ug/ml. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Description | Noelin-1 (neuronal olfactomedin-related endoplasmic reticulum-localized-1), also known as olfactomedin-1 or pancortin, is a 75kD\ secreted extracellular matrix glycoprotein expressed in the brain.1-3 Alternate promoter usage and splicing creates four forms of Noelin (Noelin 1-4) that are combinations of a common central region with alternate N and C termini.3, 4 Noelin-1 is the longest of the four isoforms, encoding a 16 amino acid (aa) signal sequence and a 469 aa mature protein with a coiled coil region for multimerization and an olfactomedin-like domain. The shortest isoforms, Noelin-3 and -4, are truncated within the coiled-coil region and lack the olfactomedin-like domain.4 In the species tested, these isoforms appear to oppose actions of the longer isoforms.5 All isoforms are capable of forming homo- or heterodimers through cysteines in their common central region.2 The C-terminus of Noelin-1 (mouse aa 482-485) contains a putative endoplasmic reticulum retention sequence that has not been included in the protein.6 Mouse Noelin-1 (aa 17-481) shares > 99%, 99%, 97%, 96% and 93% aa identity with corresponding regions of rat, human, opossum, chicken and Xenopus Noelin-1, respectively. In vivo expression patterns differ somewhat between species.1 In chick early development, Noelin-1 shows highest concentration in areas that produce neural crest cells and is produced by migrating neural crest cells.7 Overproduction of Noelin-1 prolongs neural crest cell formation.7 Noelin-1 is also expressed in mouse neural crest, but not frog.1-3 All three species express Noelin-1 later in differentiating neural tissues in the spinal cord, brain and cranial ganglia.1-3 A DNA sequence encoding mouse Noelin-1 (Met 1-Arg 481; Accession # NP_062371) was fused with a six-histidine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0. Molecular Mass: Calculated molecular mass of ~ 55.7kD. As a result of glycosylation, the recombinant mouse Noelin-1 migrates as an ~ 80-90kD protein in SDS-PAGE under reducing conditions. Activity: Measured by its ability to induce epithelial to mesenchymal transformation of MDCKII cells. Treatment of MDCKII cells with 200ng/ml of recombinant mouse Noelin-1 for 3 days is sufficient to induce the transformation of MDCKII cells. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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