Platelet Glycoprotein Ib alpha, Recombinant, Human (PGIb alpha)
Catalog No : USB-P4259-12
705.77€
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| Product name | Platelet Glycoprotein Ib alpha, Recombinant, Human (PGIb alpha) | ||
|---|---|---|---|
| Catalog No | USB-P4259-12 | ||
| Supplier’s Catalog No | P4259-12 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, NS0 cells | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -20°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥90%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: ≤1.0EU/ug (LAL) | ||
| Form | Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with sterile PBS to a concentration of 100ug/ml. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Description | Platelet glycoprotein Ib alpha chain (GPIb a), also known as CD42ba, is a 145kD type I transmembrane protein that is a member of the leucine-rich repeat (LRR) family of ligand binding proteins.1-3 It is expressed by platelets as the ligand-binding subunit of the platelet GPIb-IX-V complex.4 Human GPIb a contains a 16 amino acid (aa) signal sequence, a 489 aa extracellular domain (ECD), a 21-aa transmembrane domain, and a 100 aa cytoplasmic region. The ECD contains 8 LRRs, with # 2, 3 and 4 having been demonstrated to regulate shear-dependent adhesion to von Willebrand factor (vWF).5, 6 The LRRs are followed by a thrombin-binding anionic region that includes three sulfated tyrosines, a sialomucin domain with N- and O-linked carbohydrates, and two cysteines near the membrane that allow dimerization with GP1bb.1-6 Four human isoforms with 1 to 4 repeats of aa 398-411 within the sialomucin domain of mature GPIb a are known to exist but have unknown significance.7 The ECD of human GPIb a shares 48-51% aa identity with mouse, rat, bovine and canine GPIb a. The metalloproteinase TACE/ADAM17 constitutively and inducibly cleaves GPIb a, between Gly 480 and Val 481. This releases a soluble form called glycocalicin that circulates at ~2 ug/mL.8, 9 GPIb a binding to ligands such as thrombin, kininogen, and coagulation factors XI and XII helps to initiate platelet activation and coordinate the coagulation cascade.1, 10-12 Binding of GPIb a to vWF or thrombospondin in the plasma or matrix, vWF or P-selectin on endothelial cells, or the integrin aMb2 (MAC-1) on myeloid cells, controls response to vascular injury. A DNA sequence encoding the extracellular domain of human GPIb A (His17-Leu505; Swiss/UniProt Accession: P07359) was fused with a six-histidine tag at the C-terminus. The protein was expressed in NS0 cells line. Calculated molecular mass of ~ 54.7kD. As a result of glycosylation, recombinant human GPIb a migrates as an ~ 95-120kD protein in SDS-PAGE under reducing conditions. N Terminal Sequence Analysis: His 17 SDS-PAGE: ~95-120kD, reducing conditions Activity: Measured by its binding ability in a functional ELISA. Recombinant Human CD42b/GPIb alpha is immobilized at 0.5ug/ml (100ul/well), the concentration of Recombinant Human vWF-A2 that produces 50% optimal binding response is ~6-36ng/ml in the presence of ristocetin. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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