Olfactomedin-2, Recombinant, Human (OLFM2, OM2, neuronal olfactomedin-related endoplasmic reticulum- localized-2, Noelin-2)
Catalog No : USB-O6170-02
705.77€
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| Product name | Olfactomedin-2, Recombinant, Human (OLFM2, OM2, neuronal olfactomedin-related endoplasmic reticulum- localized-2, Noelin-2) | ||
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| Catalog No | USB-O6170-02 | ||
| Supplier’s Catalog No | O6170-02 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, Chinese Hamster Ovary cell line | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | 5575 | ||
| Storage | 4°C/-20°C | ||
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| Other names | |||
| Grade | Purified | ||
| Purity | ≥90% (SDS-PAGE) | ||
| Form | Supplied as a lyophilized powder from PBS. Carrier free. Reconstitute with 100ug/ml PBS. | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Description | Olfactomedin-2 (OLFM2, OM2), also called OlfC or neuronal olfactomedin-related endoplasmic reticulum- localized-2 (Noelin-2) i s a 50kD (predicted), secreted member of the olfactomedin/noelin family of proteins. Since Noelin-2 has also been used to designate an alternately spliced form of Noelin-1, Olfactomedin-2 is the less ambiguous name. Olfactomedin domain-containing proteins are of ten found in neural tissues. Human Olfactomedin-2 expressed sequence tags have mainly been found in the brain and eye, and RNA in the cerebellum. In zebrafish, Olfactomedin-2 expression is developmentally regulated and, like Noelin-1, may play a role in neural crest cell differentiation. The human Olfactomedin-2 cDNA encodes a 20aa signal sequence and a 454 mature protein that contains two coiled-coil regions (aa58-85 and 136-193) and an Olfactomedin domain (aa194-446). Mature human Olfactomedin-2 shares 98% aa identity with rat, bovine and equine, 97% with mouse and canine, 81% with Xenopus , and 77% with zebrafish Olfactomedin-2. No alternate splice variants have been reported for Olfactomedin-2. Polymorphisms of human Olfactomedin-2, with or without additional polymorphisms in optineurin (OPTN), have been associated with a small fraction of high intraocular pressure or open-angle glaucoma cases in Japanese patients. The family member myocilin shows stronger association with these ocular disorders, and either myocilin or Noel in-1 might heterodimerize with Olfactomedin-2. Evidence suggests that Olfactomedin-2 has evolved from Noelin-1 gene duplication, and myocilin from Olfactomedin-2 gene duplication. Source: Chinese Hamster Ovary cell line, CHO-derived. Gln21-Pro454, with a C-terminal 6-His tag. Molecular Weight: 55-75kD (SDA-PAGE, reducing conditions) Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of HEK293T human embryonic kidney cells. When 5x10er cells/well are added to human Olfactomedin-2 coated plates (1ug/ml, 100ul/well), ~50-75% will adhere after 1h at 37°C. Endotoxin: ≤1EU/ug (LAL) Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with 100ug/ml PBS. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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