Heparinase III, Recombinant, P. heparinus
Catalog No : USB-H1892-01
614.96€
0.00€
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| Product name | Heparinase III, Recombinant, P. heparinus | ||
|---|---|---|---|
| Catalog No | USB-H1892-01 | ||
| Supplier’s Catalog No | H1892-01 | ||
| Supplier | US Biologicals | ||
| Source antigen | Recombinant, E.Coli | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | |||
| Molecular weight | |||
| Storage | -70°C | ||
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| Other names | |||
| Grade | Highly Purified | ||
| Purity | ≥ 95% (SDS-PAGE, reducing conditions; 5ug protein loaded per lane; visualized by colloidal Coomassie blue staining). Endotoxin: <1.0 EU/1ug (LAL). | ||
| Form | Supplied as a sterile-filtered liquid in Tris-buffered saline containing glycerol | ||
| Reactivity life | 6 months | ||
| Note | For reserch purpose only | ||
| Description | Heparan sulfate is a sulfated glycosaminoglycan with the repeating disaccharide units of -4HexA1,4GlcNAcb1-. It is usually attached to the protein cores of proteoglycans found on the cell membrane and in the extracellular matrix where it binds to a variety of protein ligands and regulates a wide range of biological activities. Heparan sulfate has a domain structure where sulfated regions are interspaced with less or non-sulfated regions. Heparin shares the backbone structure with heparan sulfate but contains no non-sulfated regions. Heparinases are a family of lyases that release unsaturated oligosaccharides from heparin and heparan sulfate upon digestion. Heparinase I recognizes highly sulfated regions and is more specific for heparin. Heparinase II digests both heparin and heparan sulfate. Heparinase III prefers less-sulfated regions and is more active on heparan sulfate. Source: E. coli-expressed recombinant protein corresponding to Gln25-Pro659 of P. heparinus Heparinase III, with an N-terminal Met and 6-His tag Molecular Weight: Predicted: 74kD Observed: 66kD (SDS-PAGE, reducing conditions) Activity: Measured by its ability to liberate oligosaccharides from heparin. Specific Activity: >3,000 pmoles/min/ug Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | ||
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