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Categories: Biochemicals / Biochemicals

Product name			Keyhole Limpet Hemocyanin (KLH)
Catalog No			USB-209047
Supplier’s Catalog No			209047
Supplier			US Biologicals
Source antigen			Megathura crenulata
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			RT/4°C
Other names
Grade			Purified
Purity			Purified
Form			Supplied as a lyophilized powder from 5mM BES, pH6.5, 10mM MgSO4. Reconstitute with PBS to 5-10mg/ml.
Reactivity life			12 months
Note			For reserch purpose only
Description			Antibodies to small peptide/haptens are generally raised by coupling to a large carrier proteins such as KLH (Keyhole Limpet Hemocyanin). KLH mol wt ranges 3-7.5 million. It is used for carrier protein as it offers plenty of free amino, carboxyl and cysteines for coupling various peptides or haptens. Antibodies are produced to both KLH and the peptide/hapten. Anti-KLH may give non-specific signals in various immunoassays. Anti-KLH antibodies can be removed by solid phase immunoaffinity column chromatography over the KLH-Agarose affinity column or by adsorbing with KLH protein. Source: KLH, isolated from the hemolymphs of the molllusk Megathura crenulata Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.