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Categories: Biochemicals / Biochemicals

Product name			Loading Dye Buffer, Tri Color, 6X
Catalog No			USB-L3350-01
Supplier’s Catalog No			L3350-01
Supplier			US Biologicals
Source antigen
Reactivity
Cross reactivity
Applications
Molecular weight

Storage			-20°C
Other names
Grade			Molecular Biology Grade
Purity
Form			Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 0.15% orange G, 60mM EDTA, 60% glycerol.
Reactivity life			12 months
Note			For reserch purpose only
Description			Loading dye for DNA markers and samples on agarose or polyacrylamide gels. It contains three dyes for easy visual tracking. he optimized solution contains three dyes: bromophenol blue, xylene cyanol FF and orange G for visual three-color tracking of DNA migration during electrophoresis. The presence of glycerol in the solution ensures that the same sample forms a layer at the bottom of the well; the EDTA included in the solution binds divalent metal ions and inhibits metal dependent nucleases. Recommended Dilutions: Add 1:5 of L3350-01 to DNA sample; mix 1 volume of dye with 5 volumes of DNA sample. In 1% agarose gels bromophenol blue comigrates with ~300bp fragment, xylene cyanol FF - with ~4000bp fragment and Orange G co-migrates with smallest fragments, i.e. ~50 bp. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.