Imugex

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Categories: Biochemicals / Biochemicals

Product name			Purification Media, Maleimide-OvA, BioSeparation™
Catalog No			USB-P9100-60
Supplier’s Catalog No			P9100-60
Supplier			US Biologicals
Source antigen
Reactivity
Cross reactivity
Applications			IP
Molecular weight

Storage			-20°C
Other names
Grade			Molecular Biology Grade
Purity
Form
Reactivity life			12 months
Note			For reserch purpose only
Description			Activated KLH, BSA and Ovalbumin (OVA), are commonly used as protein carriers for haptens such as peptides in order to enable the immune response to small molecules. In many cases KLH conjugate is used for the immunization, while BSA or OVA conjugate is used in immunoassys of the resulting antibodies. OVA conjugates are used in order to filter out the antibodies directed to the KLH. However in some cases OVA or other proteins are preffered as carrier proteins because of a variety of other reasons. The Maleimide activated KLH and OVA are preactivated with a heterobifunctional cross-linker (GMBS). These activated proteins may be reacted with biomolecules that contain a free sulfhydryl groups, to form a stable thioether bond. Activation method: GMBS Binding Capacity: ~2-4mg of peptide (average MW of 1000-2500) per 2mg OVA Protein Concentration: 4mg/ml (0.5ml) Storage Buffer: PBS pH 7.5 Storage and Stability: Stable for 3 days refrigerated