Anti-HLA-DQA2 Antibody

Catalog No : IGX-40814
262.00€
188.00€

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Description Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
Other names HLA class II histocompatibility antigen, DQ alpha 2 chain, DX alpha chain, HLA class II histocompatibility antigen, DQ,6, alpha chain, HLA-DQA1, MHC class II DQA2, HLA-DQA2, HLA-DXA
Cellular localization Trans-Golgi Network Membrane, Single-Pass Type I Membrane, Endosome Membrane, Endoplasmic Reticulum Membrane, Membrane, Lysosome Membrane
Research area Immunology
Note This product is for research purpose only, not for use in diagnostics or as therapeutics.
Uniprot ID P01906
Product name Anti-HLA-DQA2 Antibody
Catalog No IGX-40814
Supplier’s Catalog No IGX-40814
Supplier ImuGeX
Host species Rabbit
Species reactivity Human
Applications ELISA, IHC
Recommended dilutions IHC: 1:10-1:100
Molecular weight N/A
Conjugate Unconjugated
Immunogen Recombinant Human HLA class II histocompatibility antigen, DQ alpha 2 chain protein (24-214AA)
Specificity HLA-DQA2 antibody detects endogenous level of HLA-DQA2 protein
Storage Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity.
Clonality Polyclonal
Isotype IgG
Clone ID N/A
Concentration 1 mg/mL
Storage buffer Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol.
Formulation Liquid
Purification This antibody is immunogen affinity purified.
Handling This product contains Proclin as preservative, please take appropriate care when handling this product.
Reactivity life 12 months when stored properly according to instructions (provided antibody remains sterile).