Anti-APOE / Apolipoprotein E Antibody

Catalog No : IGX-40903
262.00€
188.00€

Shipping cost plus VAT not included , delivery in 7-14 business days

Description APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids (PMID:6860692, PMID:1911868, PMID:14754908). APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance (PMID:6860692, PMID:2762297, PMID:1911868, PMID:1917954, PMID:9395455, PMID:14754908, PMID:23620513). Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma (PMID:6860692, PMID:2762297, PMID:9395455). As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL) (PMID:6860692, PMID:1911868). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles (PMID:2762297, PMID:1917954, PMID:7768901, PMID:8939961, PMID:12950167, PMID:20030366, PMID:2063194, PMID:8756331, PMID:20303980, PMID:1530612, PMID:7635945). Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells (PMID:9395455, PMID:9488694, PMID:23676495, PMID:7635945). A main function of APOE is to mediate lipoprotein clearance through the uptake of chylomicrons, VLDLs, and HDLs by hepatocytes (PMID:1911868, PMID:1917954, PMID:9395455, PMID:23676495, PMID:29516132). APOE is also involved in the biosynthesis by the liver of VLDLs as well as their uptake by peripheral tissues ensuring the delivery of triglycerides and energy storage in muscle, heart and adipose tissues (PMID:2762297, PMID:29516132). By participating in the lipoprotein-mediated distribution of lipids among tissues, APOE plays a critical role in plasma and tissues lipid homeostasis (PMID:2762297, PMID:1917954, PMID:29516132). APOE is also involved in two steps of reverse cholesterol transport, the HDLs-mediated transport of cholesterol from peripheral tissues to the liver, and thereby plays an important role in cholesterol homeostasis (PMID:9395455, PMID:14754908, PMID:23620513). First, it is functionally associated with ABCA1 in the biogenesis of HDLs in tissues (PMID:14754908, PMID:23620513). Second, it is enriched in circulating HDLs and mediates their uptake by hepatocytes (PMID:9395455). APOE also plays an important role in lipid transport in the central nervous system, regulating neuron survival and sprouting (PMID:8939961, PMID:25173806). APOE in also involved in innate and adaptive immune responses, controlling for instance the survival of myeloid-derived suppressor cells . APOE, may also play a role in transcription regulation through a receptor-dependent and cholesterol-independent mechanism, that activates MAP3K12 and a non-canonical MAPK signal transduction pathway that results in enhanced AP-1-mediated transcription of APP (PMID:28111074).
Other names Apolipoprotein E, Apo-E, APOE
Cellular localization Secreted, Extracellular Space, Extracellular Matrix
Research area Neuroscience
Note This product is for research purpose only, not for use in diagnostics or as therapeutics.
Uniprot ID P02649
Product name Anti-APOE / Apolipoprotein E Antibody
Catalog No IGX-40903
Supplier’s Catalog No IGX-40903
Supplier ImuGeX
Host species Rabbit
Species reactivity Human
Applications ELISA, IHC, IF
Recommended dilutions IHC: 1:1000-1:2000, IF: 1:200-1:500
Molecular weight N/A
Conjugate Unconjugated
Immunogen Recombinant Human Apolipoprotein E protein (21-110AA)
Specificity APOE antibody detects endogenous level of APOE protein
Storage Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity.
Clonality Polyclonal
Isotype IgG
Clone ID N/A
Concentration Concentration can vary between different batches.
Storage buffer Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol.
Formulation Liquid
Purification This antibody is immunogen affinity purified.
Handling This product contains Proclin as preservative, please take appropriate care when handling this product.
Reactivity life 12 months when stored properly according to instructions (provided antibody remains sterile).