Anti-ASAH1 Antibody
Catalog No : IGX-41938
237.00€
175.00€
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| Description | Lysosomal ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at acidic pH (PMID:10610716, PMID:7744740, PMID:15655246, PMID:11451951). Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PMID:10610716). Has a higher catalytic efficiency towards C12-ceramides versus other ceramides (PMID:7744740, PMID:15655246). Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine (PMID:12764132, PMID:12815059). For the reverse synthetic reaction, the natural sphingosine D-erythro isomer is more efficiently utilized as a substrate compared to D-erythro-dihydrosphingosine and D-erythro-phytosphingosine, while the fatty acids with chain lengths of 12 or 14 carbons are the most efficiently used (PMID:12764132). Has also an N-acylethanolamine hydrolase activity (PMID:15655246). By regulating the levels of ceramides, sphingosine and sphingosine-1-phosphate in the epidermis, mediates the calcium-induced differentiation of epidermal keratinocytes (PMID:17713573). Also indirectly regulates tumor necrosis factor/TNF-induced apoptosis . By regulating the intracellular balance between ceramides and sphingosine, in adrenocortical cells, probably also acts as a regulator of steroidogenesis (PMID:22261821). Isoform 2: May directly regulate steroidogenesis by binding the nuclear receptor NR5A1 and negatively regulating its transcriptional activity. | ||
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| Other names | Acid ceramidase, AC, ACDase, Acid CDase, EC 3.5.1.23, Acylsphingosine deacylase, N-acylsphingosine amidohydrolase, Putative 32 kDa heart protein, PHP32, [Cleaved into: Acid ceramidase subunit alpha, Acid ceramidase subunit beta], ASAH1, ASAH | ||
| Cellular localization | Cytoplasm, Lysosome, Nucleus, Secreted | ||
| Research area | Signal Transduction | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | Q13510 | ||
| Product name | Anti-ASAH1 Antibody | ||
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| Catalog No | IGX-41938 | ||
| Supplier’s Catalog No | IGX-41938 | ||
| Supplier | ImuGeX | ||
| Host species | Rabbit | ||
| Species reactivity | Human | ||
| Applications | ELISA, IHC | ||
| Recommended dilutions | IHC: 1:20-1:200 | ||
| Molecular weight | N/A | ||
| Conjugate | Unconjugated | ||
| Immunogen | Recombinant Human Acid ceramidase protein (146-395AA) | ||
| Specificity | ASAH1 antibody detects endogenous level of ASAH1 protein | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
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| Clonality | Polyclonal | ||
| Isotype | IgG | ||
| Clone ID | N/A | ||
| Concentration | Concentration can vary between different batches. | ||
| Storage buffer | Phosphate-buffered saline (pH 7.3) containing 0.02% Sodium Azide (NaN3) and 50% Glycerol. | ||
| Formulation | Liquid | ||
| Purification | This antibody is immunogen affinity purified. | ||
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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