Anti-ALKBH1 Antibody
Catalog No : IGX-41948
237.00€
175.00€
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| Description | Dioxygenase that acts as on nucleic acids, such as DNA and tRNA (PMID:18603530, PMID:27745969, PMID:27497299). Requires molecular oxygen, alpha-ketoglutarate and iron (PMID:18603530, PMID:27497299). A number of activities have been described for this dioxygenase, but recent results suggest that it mainly acts as on tRNAs and mediates their demethylation or oxidation depending on the context and subcellular compartment (PMID:27745969, PMID:27497299). Mainly acts as a tRNA demethylase by removing N1-methyladenine from various tRNAs, with a preference for N1-methyladenine at position 58 (m1A58) present on a stem loop structure of tRNAs (PMID:27745969). Acts as a regulator of translation initiation and elongation in response to glucose deprivation: regulates both translation initiation, by mediating demethylation of tRNA(Met), and translation elongation, N1-methyladenine-containing tRNAs being preferentially recruited to polysomes to promote translation elongation (PMID:27745969). In mitochondrion, specifically interacts with mt-tRNA(Met) and mediates oxidation of mt-tRNA(Met) methylated at cytosine(34) to form 5-formylcytosine (f5c) at this position (PMID:27497299). mt-tRNA(Met) containing the f5c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation (PMID:27497299). Specifically demethylates DNA methylated on the 6th position of adenine (N6-methyladenosine) DNA (PMID:30017583). N6-methyladenosine (m6A) DNA is present at some L1 elements in embryonic stem cells and probably promotes their silencing . Demethylates mRNAs containing N3-methylcytidine modification (PMID:31188562). Also able to repair alkylated single-stranded DNA by oxidative demethylation, but with low activity (PMID:18603530). Also has DNA lyase activity and introduces double-stranded breaks at abasic sites: cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites (PMID:19959401). DNA lyase activity does not require alpha-ketboglutarate and iron and leads to the formation of an irreversible covalent protein-DNA adduct with the 5' DNA product (PMID:19959401, PMID:23577621). DNA lyase activity is not required during base excision repair and class switch recombination of the immunoglobulin heavy chain during B lymphocyte activation. May play a role in placental trophoblast lineage differentiation . | ||
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| Other names | Nucleic acid dioxygenase ALKBH1, EC 1.14.11.-, Alkylated DNA repair protein alkB homolog 1, Alpha-ketoglutarate-dependent dioxygenase ABH1, DNA 6mA demethylase, DNA N6-methyl adenine demethylase, EC 1.14.11.51, DNA lyase ABH1, EC 4.2.99.18, DNA oxidative demethylase ALKBH1, EC 1.14.11.33, tRNA N1-methyl adenine demethylase, EC 1.14.11.-, ALKBH1, ABH ABH1 ALKBH | ||
| Cellular localization | Mitochondrion, Nucleus | ||
| Research area | Epigenetics & Nuclear Signaling | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | Q13686 | ||
| Product name | Anti-ALKBH1 Antibody | ||
|---|---|---|---|
| Catalog No | IGX-41948 | ||
| Supplier’s Catalog No | IGX-41948 | ||
| Supplier | ImuGeX | ||
| Host species | Rabbit | ||
| Species reactivity | Human | ||
| Applications | ELISA, IHC | ||
| Recommended dilutions | IHC: 1:20-1:200 | ||
| Molecular weight | N/A | ||
| Conjugate | Unconjugated | ||
| Immunogen | Recombinant Human Alkylated DNA repair protein alkB homolog 1 protein (110-389AA) | ||
| Specificity | ALKBH1 antibody detects endogenous level of ALKBH1 protein | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
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| Clonality | Polyclonal | ||
| Isotype | IgG | ||
| Clone ID | N/A | ||
| Concentration | Concentration can vary between different batches. | ||
| Storage buffer | Phosphate-buffered saline (pH 7.3) containing 0.02% Sodium Azide (NaN3) and 50% Glycerol. | ||
| Formulation | Liquid | ||
| Purification | This antibody is immunogen affinity purified. | ||
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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