Anti-DDX11 Antibody
Catalog No : IGX-42865
237.00€
175.00€
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| Description | DNA-dependent ATPase and ATP-dependent DNA helicase that participates in various functions in genomic stability, including DNA replication, DNA repair and heterochromatin organization as well as in ribosomal RNA synthesis (PMID:10648783, PMID:21854770, PMID:23797032, PMID:26089203, PMID:26503245). Its double-stranded DNA helicase activity requires either a minimal 5'-single-stranded tail length of approximately 15 nt (flap substrates) or 10 nt length single-stranded gapped DNA substrates of a partial duplex DNA structure for helicase loading and translocation along DNA in a 5' to 3' direction (PMID:18499658, PMID:22102414). The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended up to 500 bp by the replication protein A (RPA) or the cohesion CTF18-replication factor C (Ctf18-RFC) complex activities (PMID:18499658). Shows also ATPase- and helicase activities on substrates that mimic key DNA intermediates of replication, repair and homologous recombination reactions, including forked duplex, anti-parallel G-quadruplex and three-stranded D-loop DNA molecules (PMID:22102414, PMID:26503245). Plays a role in DNA double-strand break (DSB) repair at the DNA replication fork during DNA replication recovery from DNA damage (PMID:23797032). Recruited with TIMELESS factor upon DNA-replication stress response at DNA replication fork to preserve replication fork progression, and hence ensure DNA replication fidelity (PMID:26503245). Cooperates also with TIMELESS factor during DNA replication to regulate proper sister chromatid cohesion and mitotic chromosome segregation (PMID:17105772, PMID:18499658, PMID:20124417, PMID:23116066, PMID:23797032). Stimulates 5'-single-stranded DNA flap endonuclease activity of FEN1 in an ATP- and helicase-independent manner; and hence it may contribute in Okazaki fragment processing at DNA replication fork during lagging strand DNA synthesis (PMID:18499658). Its ability to function at DNA replication fork is modulated by its binding to long non-coding RNA (lncRNA) cohesion regulator non-coding RNA DDX11-AS1/CONCR, which is able to increase both DDX11 ATPase activity and binding to DNA replicating regions (PMID:27477908). Plays also a role in heterochromatin organization (PMID:21854770). Involved in rRNA transcription activation through binding to active hypomethylated rDNA gene loci by recruiting UBTF and the RNA polymerase Pol I transcriptional machinery (PMID:26089203). Plays a role in embryonic development and prevention of aneuploidy . Involved in melanoma cell proliferation and survival (PMID:23116066). Associates with chromatin at DNA replication fork regions (PMID:27477908). Binds to single- and double-stranded DNAs (PMID:9013641, PMID:18499658, PMID:22102414). | ||
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| Other names | ATP-dependent DNA helicase DDX11, EC 3.6.4.12, CHL1-related protein 1, hCHLR1, DEAD/H-box protein 11, Keratinocyte growth factor-regulated gene 2 protein, KRG-2, DDX11, CHL1 CHLR1 KRG2 | ||
| Cellular localization | Nucleus, Nucleolus, Cytoskeleton, Spindle Pole, Centrosome, Midbody, Chromosome | ||
| Research area | Cell Biology | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | Q96FC9 | ||
| Product name | Anti-DDX11 Antibody | ||
|---|---|---|---|
| Catalog No | IGX-42865 | ||
| Supplier’s Catalog No | IGX-42865 | ||
| Supplier | ImuGeX | ||
| Host species | Rabbit | ||
| Species reactivity | Human | ||
| Applications | ELISA, IHC | ||
| Recommended dilutions | IHC: 1:20-1:200 | ||
| Molecular weight | N/A | ||
| Conjugate | Unconjugated | ||
| Immunogen | Recombinant Human ATP-dependent DNA helicase DDX11 protein (1-220AA) | ||
| Specificity | DDX11 antibody detects endogenous level of DDX11 protein | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
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| Clonality | Polyclonal | ||
| Isotype | IgG | ||
| Clone ID | N/A | ||
| Concentration | Concentration can vary between different batches. | ||
| Storage buffer | Phosphate-buffered saline (pH 7.3) containing 0.02% Sodium Azide (NaN3) and 50% Glycerol. | ||
| Formulation | Liquid | ||
| Purification | This antibody is immunogen affinity purified. | ||
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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