Anti-LIMK1 Antibody
Catalog No : IGX-43315
262.00€
188.00€
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| Description | Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways (PMID:10436159, PMID:11832213, PMID:12807904, PMID:15660133, PMID:16230460, PMID:18028908, PMID:22328514, PMID:23633677). Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop (PMID:10436159). LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton (PMID:11832213, PMID:15660133, PMID:16230460, PMID:23633677). In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation (PMID:11832213, PMID:15660133, PMID:16230460, PMID:23633677). Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly (PMID:18028908). Stimulates axonal outgrowth and may be involved in brain development (PMID:18028908). Has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1). | ||
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| Other names | LIM domain kinase 1, LIMK-1, EC 2.7.11.1, LIMK1, LIMK | ||
| Cellular localization | Cytoskeleton, Nucleus, Cytoplasm, Lamellipodium | ||
| Research area | Neuroscience | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | P53667 | ||
| Product name | Anti-LIMK1 Antibody | ||
|---|---|---|---|
| Catalog No | IGX-43315 | ||
| Supplier’s Catalog No | IGX-43315 | ||
| Supplier | ImuGeX | ||
| Host species | Rabbit | ||
| Species reactivity | Human | ||
| Applications | ELISA, IHC | ||
| Recommended dilutions | IHC: 1:20-1:200 | ||
| Molecular weight | N/A | ||
| Conjugate | Unconjugated | ||
| Immunogen | Recombinant Human LIM domain kinase 1 protein (179-320AA) | ||
| Specificity | LIMK1 antibody detects endogenous level of LIMK1 protein | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
|---|---|---|---|
| Clonality | Polyclonal | ||
| Isotype | IgG | ||
| Clone ID | N/A | ||
| Concentration | 1 mg/mL | ||
| Storage buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. | ||
| Formulation | Liquid | ||
| Purification | This antibody is immunogen affinity purified. | ||
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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