Agarose DNA Fragments 150-1500bp
Catalog No : USB-A1010
245.98€
0.00€
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| Product name | Agarose DNA Fragments 150-1500bp | ||
|---|---|---|---|
| Catalog No | USB-A1010 | ||
| Supplier’s Catalog No | A1010 | ||
| Supplier | US Biologicals | ||
| Source antigen | Gelidium | ||
| Reactivity | |||
| Cross reactivity | |||
| Applications | Analytical DNA Gels ≤ 1500bp • PCR products • Restriction Enzyme digestion products • Mutation Analysis • Blotting Assays ≤ 600bp • For use with all buffer systems | ||
| Molecular weight | |||
| Storage | RT | ||
|---|---|---|---|
| Other names | |||
| Grade | Molecular Biology Grade | ||
| Purity | |||
| Form | White, homogeeous free flowing powder | ||
| Reactivity life | 12 months | ||
| Note | For reserch purpose only | ||
| Description | Agarose for DNA Fragments 150-1500bp is manufactured from selected algae and harvested during specific periods of growth to produce gels that are easier to prepare than the equivalent polyacrylamide gels. Manufactured specifically for primer size fragments in the range of 150-1500 base pairs and exhibits exceptionally high transparency even at high concentrations (4.5%). Applications: • Analytical DNA Gels ≤ 1500bp • PCR products • Restriction Enzyme digestion products • Mutation Analysis • Blotting Assays ≤ 600bp • For use with all buffer systems Recommended Usage (1X TAE): Concentration Molecular Weight 4% 150-600 bp 12% 500-1500 bp Source: Gelidium Form: White, homogeeous free flowing powder Clarity (NTU, 1.5%): ~1.5 Colorimetry (A450): ≤ 0.02 pH in solution (1.5%): 5.5-7.0 pH in gel (1.5%): 5.5-7.0 Gel Strength (1.5%): ≥2000g/cm2 Gel Strength (4%): ≥4250g/cm2 Gel Point (4%): ≤40°C Melting Point (4%): ≤89ºC EEO: ≤0.12 Loss on Drying: ≤7.0% Ash: ≤0.35% Sulfate: ≤0.1% RNase: None Detected DNase: None Detected Quality Control: Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility. Functional and Molecular Biology Testing: To Pass Test • Comparative assay of different size DNA fragments • Southern Blot (125-23,000bp) • Background Fluorescence (TAE/TE, EtBr): • DNA Binding (TAE, EtBr) • Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase) Storage and Stability: Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use. | ||
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