Anti-PICALM / CALM Antibody
Catalog No : IGX-45571
262.00€
188.00€
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| Description | Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly (PMID:10436022, PMID:16262731, PMID:27574975). Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature (PMID:25898166). In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8 (PMID:22118466, PMID:21808019, PMID:23741335). In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors (PMID:25241929). Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF) (PMID:25241929, PMID:24067654). | ||
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| Other names | Phosphatidylinositol-binding clathrin assembly protein, Clathrin assembly lymphoid myeloid leukemia protein | ||
| Cellular localization | Golgi Apparatus, Nucleus, Plasma Membrane, Membrane, Clathrin-Coated Pit, Clathrin-Coated Vesicle | ||
| Research area | Neuroscience | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | Q13492 | ||
| Product name | Anti-PICALM / CALM Antibody | ||
|---|---|---|---|
| Catalog No | IGX-45571 | ||
| Supplier’s Catalog No | IGX-45571 | ||
| Supplier | ImuGeX | ||
| Host species | Rabbit | ||
| Species reactivity | Human | ||
| Applications | ELISA, IHC, IF | ||
| Recommended dilutions | IHC: 1:500-1:1000, IF: 1:200-1:500 | ||
| Molecular weight | 71 KDa | ||
| Conjugate | Unconjugated | ||
| Immunogen | Recombinant Human Phosphatidylinositol-binding clathrin assembly protein (517-624AA) | ||
| Specificity | PICALM antibody detects endogenous level of PICALM protein | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
|---|---|---|---|
| Clonality | Polyclonal | ||
| Isotype | IgG | ||
| Clone ID | N/A | ||
| Concentration | 1 mg/mL | ||
| Storage buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. | ||
| Formulation | Liquid | ||
| Purification | This antibody is immunogen affinity purified. | ||
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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