Anti-SIAH2 Antibody
Catalog No : IGX-45576
262.00€
188.00€
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| Description | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins (PMID:9334332, PMID:11483518, PMID:19224863). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PMID:9334332, PMID:11483518, PMID:19224863). Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes (PMID:9334332, PMID:11483518, PMID:19224863). Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (GPS2, POU2AF1, PML, NCOR1), a cell surface receptor (DCC), an antiapoptotic protein (BAG1), and a protein involved in synaptic vesicle function in neurons (SYP) (PMID:9334332, PMID:11483518, PMID:19224863). Mediates ubiquitination and proteasomal degradation of DYRK2 in response to hypoxia (PMID:22878263). It is thereby involved in apoptosis, tumor suppression, cell cycle, transcription and signaling processes (PMID:9334332, PMID:11483518, PMID:19224863, PMID:22878263). Has some overlapping function with SIAH1 (PMID:9334332, PMID:11483518, PMID:19224863). Triggers the ubiquitin-mediated degradation of TRAF2, whereas SIAH1 does not (PMID:12411493). Promotes monoubiquitination of SNCA (PMID:19224863). Regulates cellular clock function via ubiquitination of the circadian transcriptional repressors NR1D1 and NR1D2 leading to their proteasomal degradation (PMID:26392558). Plays an important role in mediating the rhythmic degradation/clearance of NR1D1 and NR1D2 contributing to their circadian profile of protein abundance (PMID:26392558). Mediates ubiquitination and degradation of EGLN2 and EGLN3 in response to the unfolded protein response (UPR), leading to their degradation and subsequent stabilization of ATF4 . | ||
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| Other names | E3 ubiquitin-protein ligase SIAH2, EC 2.3.2.27, RING-type E3 ubiquitin transferase SIAH2, Seven in absentia homolog 2, Siah-2, hSiah2, SIAH2 | ||
| Cellular localization | Nucleus, Cytoplasm | ||
| Research area | Neuroscience | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | O43255 | ||
| Product name | Anti-SIAH2 Antibody | ||
|---|---|---|---|
| Catalog No | IGX-45576 | ||
| Supplier’s Catalog No | IGX-45576 | ||
| Supplier | ImuGeX | ||
| Host species | Rabbit | ||
| Species reactivity | Human | ||
| Applications | ELISA, IHC, IF | ||
| Recommended dilutions | IHC: 1:500-1:1000, IF: 1:200-1:500 | ||
| Molecular weight | N/A | ||
| Conjugate | Unconjugated | ||
| Immunogen | Recombinant Human E3 ubiquitin-protein ligase SIAH2 protein (1-72AA) | ||
| Specificity | SIAH2 antibody detects endogenous level of SIAH2 protein | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
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| Clonality | Polyclonal | ||
| Isotype | IgG | ||
| Clone ID | N/A | ||
| Concentration | 1 mg/mL | ||
| Storage buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. | ||
| Formulation | Liquid | ||
| Purification | This antibody is immunogen affinity purified. | ||
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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