Anti-Ripk1 Antibody
Catalog No : IGX-45677
262.00€
188.00€
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| Description | Serine-threonine kinase which is a key regulator of TNF-mediated apoptosis, necroptosis and inflammatory pathways (PMID:25459879). Exhibits kinase activity-dependent functions that trigger cell death and kinase-independent scaffold functions regulating inflammatory signaling and cell survival (PMID:31519887, PMID:31519886). Initiates ripoptocide which describes cell death that is dependent on RIPK1, be it apoptosis or necroptosis (PMID:31457011). Upon binding of TNF to TNFR1, RIPK1 is recruited to the TNF-R1 signaling complex (TNF-RSC also known as complex I) where it acts as a scaffold protein promoting cell survival, in part, by activating the canonical NF-kB pathway (PMID:31519887, PMID:31519886). Specific conditions can however activate RIPK1, and its kinase activity then regulates assembly of two death-inducing complexes, namely complex IIa (RIPK1-FADD-CASP8) and the complex IIb (RIPK1-RIPK3-MLKL) and these complexes respectively drive apoptosis or necroptosis, a regulated form of necrosis (PMID:29440439, PMID:30988283, PMID:31519887, PMID:31519886). Also involved in inflammatory response by promoting transcriptional production of pro-inflammatory cytokines, such as interleukin-6 (IL6) (PMID:31827281, PMID:31827280). During embryonic development suppresses apoptosis and necroptosis and prevents the interaction of TRADD with FADD thereby limiting aberrant activation of CASP8 (PMID:30867408, PMID:30185824, PMID:31511692). Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade . Required for ZBP1-induced NF-kappa-B activation and activation of NF-kappa-B by DNA damage and IR (PMID:12654725, PMID:19590578). | ||
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| Other names | Receptor-interacting serine/threonine-protein kinase 1, EC 2.7.11.1, Cell death protein RIP, Receptor-interacting protein 1, RIP-1, Serine/threonine-protein kinase RIP, Ripk1, Rinp Rip | ||
| Cellular localization | Membrane, Cytoplasm | ||
| Research area | Cell Biology | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | Q60855 | ||
| Product name | Anti-Ripk1 Antibody | ||
|---|---|---|---|
| Catalog No | IGX-45677 | ||
| Supplier’s Catalog No | IGX-45677 | ||
| Supplier | ImuGeX | ||
| Host species | Rabbit | ||
| Species reactivity | Mouse, Human | ||
| Applications | ELISA, IP | ||
| Recommended dilutions | IP: 1:200-1:2000 | ||
| Molecular weight | N/A | ||
| Conjugate | Unconjugated | ||
| Immunogen | Recombinant Mouse Receptor-interacting serine/threonine-protein kinase 1 protein (1-656AA) | ||
| Specificity | Ripk1 antibody detects endogenous level of Ripk1 protein | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
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| Clonality | Polyclonal | ||
| Isotype | IgG | ||
| Clone ID | N/A | ||
| Concentration | 1 mg/mL | ||
| Storage buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. | ||
| Formulation | Liquid | ||
| Purification | This antibody is immunogen affinity purified. | ||
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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