Anti-Samhd1 Antibody
Catalog No : IGX-45997
262.00€
188.00€
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| Description | Isoform 1: Protein that acts both as a host restriction factor involved in defense response to virus and as a regulator of DNA end resection at stalled replication forks . Has deoxynucleoside triphosphate (dNTPase) activity, which is required to restrict infection by viruses: dNTPase activity reduces cellular dNTP levels to levels too low for retroviral reverse transcription to occur, blocking early-stage virus replication in dendritic and other myeloid cells (PMID:23972988, PMID:23872947, PMID:26667483, PMID:29379009). Likewise, suppresses LINE-1 retrotransposon activity (PMID:26667483). In addition to virus restriction, dNTPase activity acts as a regulator of DNA precursor pools by regulating dNTP pools . Phosphorylation at Thr-634 acts as a switch to control dNTPase-dependent and -independent functions: it inhibits dNTPase activity and ability to restrict infection by viruses, while it promotes DNA end resection at stalled replication forks . Functions during S phase at stalled DNA replication forks to promote the resection of gapped or reversed forks: acts by stimulating the exonuclease activity of MRE11, activating the ATR-CHK1 pathway and allowing the forks to restart replication . Its ability to promote degradation of nascent DNA at stalled replication forks is required to prevent induction of type I interferons, thereby preventing chronic inflammation . Ability to promote DNA end resection at stalled replication forks is independent of dNTPase activity . Enhances immunoglobulin hypermutation in B-lymphocytes by promoting transversion mutation (PMID:29669924). | ||
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| Other names | Deoxynucleoside triphosphate triphosphohydrolase SAMHD1, dNTPase, EC 3.1.5.-, Interferon-gamma-inducible protein Mg11, SAM domain and HD domain-containing protein 1, Samhd1, Mg11 | ||
| Cellular localization | Chromosome, Nucleus | ||
| Research area | Cell Biology | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | Q60710 | ||
| Product name | Anti-Samhd1 Antibody | ||
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| Catalog No | IGX-45997 | ||
| Supplier’s Catalog No | IGX-45997 | ||
| Supplier | ImuGeX | ||
| Host species | Rabbit | ||
| Species reactivity | Mouse, Human | ||
| Applications | ELISA, WB | ||
| Recommended dilutions | WB: 1:1000-1:5000 | ||
| Molecular weight | Predicted band size: 76 KDa. Observed band size: 76 KDa. | ||
| Conjugate | Unconjugated | ||
| Immunogen | Recombinant Mouse Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 protein (426-657AA) | ||
| Specificity | Samhd1 antibody detects endogenous level of Samhd1 protein | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
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| Clonality | Polyclonal | ||
| Isotype | IgG | ||
| Clone ID | N/A | ||
| Concentration | 1 mg/mL | ||
| Storage buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. | ||
| Formulation | Liquid | ||
| Purification | This antibody is immunogen affinity purified. | ||
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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