Anti-sdeA Antibody
Catalog No : IGX-46297
262.00€
188.00€
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| Description | Secreted effector that interferes with the host cell ubiquitin pathway and is required for intracellular bacterial replication. Catalyzes the ubiquitination of several mammalian Rab proteins (Rab33b, Rab1, Rab6a and Rab30) during L.pneumophila infection, without engaging the standard cellular enzyme cascade (E1 and E2) (PMID:29795346). Transfers an ADP-ribose moiety from NAD to the 'Arg-42' residue of ubiquitin in a reaction that releases nicotinamide (PMID:31330532, PMID:31330531, PMID:29795347). The modified ubiquitin is subsequently transferred to serine residues of the substrate protein via a phosphoribose linker that results in the release of AMP (PMID:27912065). Cannot ubiquitinate the endosomal Rab5 or the cytoskeletal small GTPase Rac1 (PMID:27049943). Also acts as a deubiquitinase (DUB), catalyzing the cleavage of three of the most abundant polyubiquitin chains ('Lys-11', 'Lys-48' and 'Lys-63') with a distinct preference for 'Lys-63' linkages; is thus able to efficiently remove 'Lys-63'-linked polyubiquitin chains from the phagosomal surface. Is also able to remove NEDD8 from neddylated proteins, but is unable to recognize SUMO. The DUB activity of SdeA is important for regulating the dynamics of ubiquitin association with the bacterial phagosome, but is dispensable for its role in intracellular bacterial replication (PMID:26598703). | ||
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| Other names | Ubiquitinating/deubiquitinating enzyme SdeA, Effector protein SdeA, [Includes: Deubiquitinase, DUB, EC 3.4.22.-, Deneddylase, Deubiquitinating enzyme, Ubiquitin transferase, EC 2.3.2.-, Mono-ADP-ribosyltransferase, mART, EC 2.4.2.31,], sdeA | ||
| Cellular localization | Secreted, Host Cell | ||
| Research area | Cell Biology | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | Q5ZTK4 | ||
| Product name | Anti-sdeA Antibody | ||
|---|---|---|---|
| Catalog No | IGX-46297 | ||
| Supplier’s Catalog No | IGX-46297 | ||
| Supplier | ImuGeX | ||
| Host species | Rabbit | ||
| Species reactivity | Legionella pneumophila subsp | ||
| Applications | ELISA, WB | ||
| Recommended dilutions | WB: 1:1000-1:5000 | ||
| Molecular weight | Predicted band size: 32 KDa. Observed band size: 32 KDa. | ||
| Conjugate | Unconjugated | ||
| Immunogen | Recombinant Legionella pneumophila subsp Ubiquitinating/deubiquitinating enzyme SdeA protein (19-268AA) | ||
| Specificity | sdeA antibody detects endogenous level of sdeA protein | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
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| Clonality | Polyclonal | ||
| Isotype | IgG | ||
| Clone ID | N/A | ||
| Concentration | Concentration can vary between different batches. | ||
| Storage buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. | ||
| Formulation | Liquid | ||
| Purification | This antibody is immunogen affinity purified. | ||
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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