Anti-FEN1 / Flap endonuclease 1 Antibody
Catalog No : IGX-46314
262.00€
188.00€
Shipping cost plus VAT not included , delivery in 7-14 business days
| Description | Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. | ||
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| Other names | Flap endonuclease 1, FEN-1, Flap structure-specific endonuclease 1, FEN1 | ||
| Cellular localization | Nucleus, Nucleolus, Nucleoplasm, Mitochondrion | ||
| Research area | Epigenetics & Nuclear Signaling | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | O65251 | ||
| Product name | Anti-FEN1 / Flap endonuclease 1 Antibody | ||
|---|---|---|---|
| Catalog No | IGX-46314 | ||
| Supplier’s Catalog No | IGX-46314 | ||
| Supplier | ImuGeX | ||
| Host species | Rabbit | ||
| Species reactivity | Arabidopsis thaliana | ||
| Applications | ELISA, WB | ||
| Recommended dilutions | WB: 1:1000-1:5000 | ||
| Molecular weight | Predicted band size: 37 KDa. Observed band size: 45 KDa. | ||
| Conjugate | Unconjugated | ||
| Immunogen | Recombinant Arabidopsis thaliana Flap endonuclease 1 protein (85-383AA) | ||
| Specificity | FEN1 antibody detects endogenous level of FEN1 protein | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
|---|---|---|---|
| Clonality | Polyclonal | ||
| Isotype | IgG | ||
| Clone ID | N/A | ||
| Concentration | Concentration can vary between different batches. | ||
| Storage buffer | Phosphate-buffered saline (pH 7.4) containing 0.03% Proclin and 50% Glycerol. | ||
| Formulation | Liquid | ||
| Purification | This antibody is immunogen affinity purified. | ||
| Handling | This product contains Proclin as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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