Anti-CYP27A1 / Sterol 26-hydroxylase mitochondrial Antibody
Catalog No : IGX-52883
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| Description | Cytochrome P450 monooxygenase that catalyzes regio- and stereospecific hydroxylation of cholesterol and its derivatives. Hydroxylates (with R stereochemistry) the terminal methyl group of cholesterol side-chain in a three step reaction to yield at first a C26 alcohol, then a C26 aldehyde and finally a C26 acid (PMID:9660774, PMID:12077124, PMID:21411718, PMID:28190002). Regulates cholesterol homeostasis by catalyzing the conversion of excess cholesterol to bile acids via both the 'neutral' (classic) and the 'acid' (alternative) pathways (PMID:9660774, PMID:1708392, PMID:11412116, PMID:2019602, PMID:7915755, PMID:9186905, PMID:9790667). May also regulate cholesterol homeostasis via generation of active oxysterols, which act as ligands for NR1H2 and NR1H3 nuclear receptors, modulating the transcription of genes involved in lipid metabolism (PMID:9660774, PMID:12077124). Plays a role in cholestanol metabolism in the cerebellum. Similarly to cholesterol, hydroxylates cholestanol and may facilitate sterol diffusion through the blood-brain barrier to the systemic circulation for further degradation (PMID:28190002). Also hydroxylates retinal 7-ketocholesterol, a noxious oxysterol with pro-inflammatory and pro-apoptotic effects, and may play a role in its elimination from the retinal pigment epithelium (PMID:21411718). May play a redundant role in vitamin D biosynthesis. Catalyzes 25-hydroxylation of vitamin D3 that is required for its conversion to a functionally active form (PMID:15465040). | ||
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| Other names | Cytochrome P450, family 27, subfamily A, polypeptide 1 | ||
| Cellular localization | Mitochondrion Inner Membrane, Peripheral Membrane | ||
| Research area | Cardiovascular | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | Q02318 | ||
| Product name | Anti-CYP27A1 / Sterol 26-hydroxylase mitochondrial Antibody | ||
|---|---|---|---|
| Catalog No | IGX-52883 | ||
| Supplier’s Catalog No | IGX-52883 | ||
| Supplier | ImuGeX | ||
| Host species | Rabbit | ||
| Species reactivity | Human, Mouse | ||
| Applications | ELISA, WB, IHC | ||
| Recommended dilutions | ELISA: 1:1000-1:2000, WB: 1:200-1:1000, IHC: 1:25-1:100 | ||
| Molecular weight | |||
| Conjugate | Unconjugated | ||
| Immunogen | Fusion protein of human CYP27A1 | ||
| Specificity | CYP27A1 antibody detects endogenous level of CYP27A1 protein | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
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| Clonality | Polyclonal | ||
| Isotype | IgG | ||
| Clone ID | N/A | ||
| Concentration | Concentration can vary between different batches. | ||
| Storage buffer | Phosphate-buffered saline (pH 7.4) containing 0.05% NaN3, 40% Glycerol | ||
| Formulation | Liquid | ||
| Purification | Affinity Purified | ||
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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