Anti-JMJD6 Antibody Monoclonal
Catalog No : IGX-58805
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| Description | Dioxygenase that can both act as a arginine demethylase and a lysyl-hydroxylase (PMID:24498420, PMID:17947579, PMID:20684070, PMID:21060799, PMID:22189873). Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Regulates RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65 (PMID:19574390). Hydroxylates its own N-terminus, which is required for homooligomerization (PMID:22189873). In addition to peptidyl-lysine 5-dioxygenase activity, may act as an RNA hydroxylase, as suggested by its ability to bind single strand RNA (PMID:20679243, PMID:29176719). Also acts as an arginine demethylase which preferentially demethylates asymmetric dimethylation (PMID:17947579, PMID:24498420, PMID:24360279). Demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), including mono-, symmetric di- and asymmetric dimethylated forms, thereby playing a role in histone code (PMID:17947579, PMID:24360279). However, histone arginine demethylation may not constitute the primary activity in vivo (PMID:17947579, PMID:21060799, PMID:22189873). In collaboration with BRD4, interacts with the positive transcription elongation factor b (P-TEFb) complex in its active form to regulate polymerase II promoter-proximal pause release for transcriptional activation of a large cohort of genes. On distal enhancers, so called anti-pause enhancers, demethylates both histone H4R3me2 and the methyl cap of 7SKsnRNA leading to the dismissal of the 7SKsnRNA:HEXIM1 inhibitor complex. After removal of repressive marks, the complex BRD4:JMJD6 attract and retain the P-TEFb complex on chromatin, leading to its activation, promoter-proximal polymerase II pause release, and transcriptional activation (PMID:24360279). Demethylates other arginine methylated-proteins such as ESR1 (PMID:24498420). Has no histone lysine demethylase activity (PMID:21060799). Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65 . Seems to be necessary for the regulation of macrophage cytokine responses (PMID:15622002). | ||
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| Other names | bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 lysyl-hydroxylase JMJD6 phosphatidylserine receptor jmjC domain-containing protein 6 histone arginine demethylase JMJD6 peptide-lysine 5-dioxygenase JMJD6 jumonji doma | ||
| Cellular localization | Nucleoplasm, Nucleolus, Cytoplasm | ||
| Research area | Epigenetics & Nuclear Signaling, Cell biology, Developmental biology, Immunology | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | Q6NYC1 | ||
| Product name | Anti-JMJD6 Antibody Monoclonal | ||
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| Catalog No | IGX-58805 | ||
| Supplier’s Catalog No | IGX-58805 | ||
| Supplier | ImuGeX | ||
| Host species | Mouse | ||
| Species reactivity | Human, Dog, Mouse, Rat | ||
| Applications | ELISA, WB, ICC | ||
| Recommended dilutions | WB: 1:500-1:5000, ICC: 1:50-1:200 | ||
| Molecular weight | |||
| Conjugate | Unconjugated | ||
| Immunogen | Recombinant fragment, corresponding to amino acids 11-248aa of human JMJD6(N-terminus). | ||
| Specificity | This antibody detects total endogenous levels of JMJD6 protein | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
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| Clonality | Monoclonal | ||
| Isotype | IgG1 | ||
| Clone ID | N/A | ||
| Concentration | 1 mg/mL | ||
| Storage buffer | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol | ||
| Formulation | Liquid | ||
| Purification | Affinity Purified | ||
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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