Anti-GPC3 / Glypican-3 Antibody
Catalog No : IGX-59416
282.00€
201.00€
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| Description | Cell surface proteoglycan that bears heparan sulfate (PMID:14610063). Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins . Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation . Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands (PMID:16227623, PMID:24496449). Positively regulates the non-canonical Wnt signaling pathway . Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression . Inhibits the dipeptidyl peptidase activity of DPP4 (PMID:17549790). Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4 . Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis . Required for coronary vascular development . Plays a role in regulating cell movements during gastrulation . | ||
|---|---|---|---|
| Other names | Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7, Secreted glypican-3, GPC3, OCI5 | ||
| Cellular localization | Membrane, Gpi-Anchor, Extracellular Side | ||
| Research area | Signal Transduction | ||
| Note | This product is for research purpose only, not for use in diagnostics or as therapeutics. | ||
| Uniprot ID | P51654 | ||
| Product name | Anti-GPC3 / Glypican-3 Antibody | ||
|---|---|---|---|
| Catalog No | IGX-59416 | ||
| Supplier’s Catalog No | IGX-59416 | ||
| Supplier | ImuGeX | ||
| Host species | Rabbit | ||
| Species reactivity | Human | ||
| Applications | ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200 | ||
| Recommended dilutions | WB: 1:500-1:5000, IF: 1:20-1:200 | ||
| Molecular weight | Predicted band size: 66 KDa Observed band size: 66 KDa | ||
| Conjugate | Unconjugated | ||
| Immunogen | A synthesized peptide derived from human GPC3 | ||
| Specificity | This antibody detects total endogenous levels of GPC3 protein | ||
| Storage | Store at +4°C for short term (1 week), for long term aliquote and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles, with each freeze and thaw cycle an antibody can lose half of its binding activity. | ||
|---|---|---|---|
| Clonality | Monoclonal | ||
| Isotype | Rabbit IgG | ||
| Clone ID | N/A | ||
| Concentration | Concentration can vary between different batches. | ||
| Storage buffer | Phosphate-buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | ||
| Formulation | Liquid | ||
| Purification | Affinity Purified | ||
| Handling | This product contains sodium azide (NaN3) as preservative, please take appropriate care when handling this product. | ||
| Reactivity life | 12 months when stored properly according to instructions (provided antibody remains sterile). | ||
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